Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Patharapol Piamsomboon; Seong Kyoon Choi; Bambang Hanggono; Yani Lestari Nuraini; Fatma Wati; Kathy F.J. Tang; Song Park; Dongmi Kwak; Man Hee Rhee; Jee Eun Han; Ji Hyung Kim

doi:10.3390/pathogens8040233

Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Patharapol Piamsomboon, Seong Kyoon Choi, Bambang Hanggono, Yani Lestari Nuraini, Fatma Wati, Kathy F.J. Tang, Song Park, Dongmi Kwak, Man Hee Rhee, Jee Eun Han, Ji Hyung Kim

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10⁶–10⁸ copies mg⁻¹), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10¹–10³ copies mg⁻¹ or mL⁻¹ of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

Original language	English (US)
Article number	233
Journal	Pathogens
Volume	8
Issue number	4
DOIs	https://doi.org/10.3390/pathogens8040233
State	Published - Dec 2019

Keywords

Enterocytozoon hepatopenaei
Microsporidian
QPCR
Shrimp parasite

ASJC Scopus subject areas

Immunology and Allergy
Molecular Biology
General Immunology and Microbiology
Microbiology (medical)
Infectious Diseases

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10.3390/pathogens8040233

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Piamsomboon, P., Choi, S. K., Hanggono, B., Nuraini, Y. L., Wati, F., Tang, K. F. J., Park, S., Kwak, D., Rhee, M. H., Han, J. E., & Kim, J. H. (2019). Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR. Pathogens, 8(4), Article 233. https://doi.org/10.3390/pathogens8040233

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title = "Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR",

abstract = "We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.",

keywords = "Enterocytozoon hepatopenaei, Microsporidian, QPCR, Shrimp parasite",

author = "Patharapol Piamsomboon and Choi, {Seong Kyoon} and Bambang Hanggono and Nuraini, {Yani Lestari} and Fatma Wati and Tang, {Kathy F.J.} and Song Park and Dongmi Kwak and Rhee, {Man Hee} and Han, {Jee Eun} and Kim, {Ji Hyung}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = dec,

doi = "10.3390/pathogens8040233",

language = "English (US)",

volume = "8",

journal = "Pathogens",

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AU - Piamsomboon, Patharapol

AU - Choi, Seong Kyoon

AU - Hanggono, Bambang

AU - Nuraini, Yani Lestari

AU - Wati, Fatma

AU - Tang, Kathy F.J.

AU - Park, Song

AU - Kwak, Dongmi

AU - Rhee, Man Hee

AU - Han, Jee Eun

AU - Kim, Ji Hyung

PY - 2019/12

Y1 - 2019/12

N2 - We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

AB - We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

KW - Enterocytozoon hepatopenaei

KW - Microsporidian

KW - QPCR

KW - Shrimp parasite

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ER -

Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Abstract

Keywords

ASJC Scopus subject areas

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