@inbook{f2b88161c19f43408b8be8d9d03dc21b,
title = "Quantification of double-strand breaks in mammalian cells using pulsed-field gel electrophoresis",
abstract = "The double-strand break (DSB) is the most cytotoxic type of DNA damage and measurement of DSBs in cells is essential to understand their induction and repair. Pulsed-field gel electrophoresis (PFGE) allows for quantitative measurement of DSBs in a cell population generated by DNA damaging agents. PFGE has the capacity to separate DNA molecules from several hundred base pairs to over six million base pairs. In the method described here, molecules from five hundred thousand to three million base pairs are consolidated into a single band on the gel that is readily analyzed.",
keywords = "Agarose gel, DNA damage, Double-strand breaks, Intercalating agent, Pulsed-field gel electrophoresis, Radiation",
author = "Pond, {Kelvin W.} and Ellis, {Nathan A.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9500-4_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "75--85",
booktitle = "Methods in Molecular Biology",
}