pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Andrew D. Johnson; Paul A. Krieg

doi:10.1016/0378-1119(94)90070-1

pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Andrew D. Johnson
, Paul A. Krieg

Cellular & Molecular Medicine

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

Original language	English (US)
Pages (from-to)	223-226
Number of pages	4
Journal	Gene
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90070-1
State	Published - Sep 30 1994

Keywords

EF-1α promoter
Plasmid
blastula
cat reporter
micro-injection
tadpole

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90070-1

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title = "pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos",

abstract = "We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.",

keywords = "EF-1α promoter, Plasmid, blastula, cat reporter, micro-injection, tadpole",

author = "Johnson, \{Andrew D.\} and Krieg, \{Paul A.\}",

note = "Funding Information: A.D.J. was supported by an NIH Developmental Biology training grant and by an NRSA from the NIH. This work was supported by NIH grant HD2.5179 awarded to P.A.K.",

year = "1994",

month = sep,

day = "30",

doi = "10.1016/0378-1119(94)90070-1",

language = "English (US)",

volume = "147",

pages = "223--226",

journal = "Gene",

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TY - JOUR

T1 - pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

AU - Johnson, Andrew D.

AU - Krieg, Paul A.

N1 - Funding Information: A.D.J. was supported by an NIH Developmental Biology training grant and by an NRSA from the NIH. This work was supported by NIH grant HD2.5179 awarded to P.A.K.

PY - 1994/9/30

Y1 - 1994/9/30

N2 - We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

AB - We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

KW - EF-1α promoter

KW - Plasmid

KW - blastula

KW - cat reporter

KW - micro-injection

KW - tadpole

UR - https://www.scopus.com/pages/publications/0028169246

UR - https://www.scopus.com/pages/publications/0028169246#tab=citedBy

U2 - 10.1016/0378-1119(94)90070-1

DO - 10.1016/0378-1119(94)90070-1

M3 - Article

C2 - 7926804

AN - SCOPUS:0028169246

SN - 0378-1119

VL - 147

SP - 223

EP - 226

JO - Gene

JF - Gene

IS - 2

ER -

pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Abstract

Keywords

ASJC Scopus subject areas

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