TY - JOUR
T1 - Purification and characterization of stage-specific glycoproteins from Trypanosoma cruzi
AU - Harth, Günter
AU - Haidaris, Constantine G.
AU - So, Magdalene
N1 - Funding Information:
This work was supported in part by the Rockefeller Foundation and National Institutes of Health Grant AI 22983 (M.S.), by National Institutes of Health National Research Service Award AI 06837 (C.G.H.), and by Grant Ha 1312/1-1 from the Deutsche Forschungsgemein-schaft (G.H.).
PY - 1989/3/1
Y1 - 1989/3/1
N2 - Four developmentally regulated glycoproteins were purified from detergent solubilized cell membranes of Trypanosoma cruzi. Three trypomastigote specific glycoproteins each migrated as single bands under denaturing conditions with approximate Mr of 90 000, 85 000, and 55 000 and pI values of 4.3-5.0, 8.5-9.1, and 8.2, respectively. The fourth, epimastigote specific, protein had an approximate Mr of 72 000 and a pI of 4.8-5.1. The Mr of all four glycoproteins changed by 5-50% upon endoglycosidase F treatment. The Mr 72 000 antigen was the only one that reacted strongly with anti-epimastigote sera raised in rabbits. Sera from a Chagasic patient reacted strongly with the three trypomastigote specific glycoproteins and very weakly with the Mr 72 000 glycoprotein.
AB - Four developmentally regulated glycoproteins were purified from detergent solubilized cell membranes of Trypanosoma cruzi. Three trypomastigote specific glycoproteins each migrated as single bands under denaturing conditions with approximate Mr of 90 000, 85 000, and 55 000 and pI values of 4.3-5.0, 8.5-9.1, and 8.2, respectively. The fourth, epimastigote specific, protein had an approximate Mr of 72 000 and a pI of 4.8-5.1. The Mr of all four glycoproteins changed by 5-50% upon endoglycosidase F treatment. The Mr 72 000 antigen was the only one that reacted strongly with anti-epimastigote sera raised in rabbits. Sera from a Chagasic patient reacted strongly with the three trypomastigote specific glycoproteins and very weakly with the Mr 72 000 glycoprotein.
KW - Stage-specific glycoprotein
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=0024596066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024596066&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(89)90028-5
DO - 10.1016/0166-6851(89)90028-5
M3 - Article
C2 - 2498655
AN - SCOPUS:0024596066
SN - 0166-6851
VL - 33
SP - 143
EP - 150
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -