Protocol to record and quantify the intracellular pH in contracting cardiomyocytes

Yankun Lyu; Valeriy Timofeyev; James Overton; Phung N. Thai; Ebenezer N. Yamoah; Nipavan Chiamvimonvat; Xiao Dong Zhang

doi:10.1016/j.xpro.2022.101301

Protocol to record and quantify the intracellular pH in contracting cardiomyocytes

Yankun Lyu
, Valeriy Timofeyev
, James Overton
, Phung N. Thai
, Ebenezer N. Yamoah
, Nipavan Chiamvimonvat
, Xiao Dong Zhang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Intracellular pH (pH_i) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pH_i measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pH_i and sarcomere length in contracting cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).

Original language	English (US)
Article number	101301
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101301
State	Published - Jun 17 2022
Externally published	Yes

Keywords

Cell isolation
Cell-based Assays
Metabolism
Microscopy
Single Cell

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.101301

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title = "Protocol to record and quantify the intracellular pH in contracting cardiomyocytes",

abstract = "Intracellular pH (pHi) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pHi measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pHi and sarcomere length in contracting cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).",

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AU - Lyu, Yankun

AU - Timofeyev, Valeriy

AU - Overton, James

AU - Thai, Phung N.

AU - Yamoah, Ebenezer N.

AU - Chiamvimonvat, Nipavan

AU - Zhang, Xiao Dong

PY - 2022/6/17

Y1 - 2022/6/17

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AB - Intracellular pH (pHi) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pHi measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pHi and sarcomere length in contracting cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).

KW - Cell isolation

KW - Cell-based Assays

KW - Metabolism

KW - Microscopy

KW - Single Cell

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JO - STAR Protocols

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Protocol to record and quantify the intracellular pH in contracting cardiomyocytes

Abstract

Keywords

ASJC Scopus subject areas

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