Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

Ole Kristian Brandtzaeg; Elin Johnsen; Hanne Roberg-Larsen; Knut Fredrik Seip; Evan L. MacLean; Laurence R. Gesquiere; Siri Leknes; Elsa Lundanes; Steven Ray Wilson

doi:10.1038/srep31693

Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

Ole Kristian Brandtzaeg, Elin Johnsen, Hanne Roberg-Larsen, Knut Fredrik Seip, Evan L. MacLean, Laurence R. Gesquiere, Siri Leknes, Elsa Lundanes, Steven Ray Wilson

Anthropology, School of

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Abstract

The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.

Original language	English (US)
Article number	31693
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep31693
State	Published - Aug 16 2016

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title = "Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum",

abstract = "The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.",

author = "Brandtzaeg, {Ole Kristian} and Elin Johnsen and Hanne Roberg-Larsen and Seip, {Knut Fredrik} and MacLean, {Evan L.} and Gesquiere, {Laurence R.} and Siri Leknes and Elsa Lundanes and Wilson, {Steven Ray}",

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AU - Brandtzaeg, Ole Kristian

AU - Johnsen, Elin

AU - Roberg-Larsen, Hanne

AU - Seip, Knut Fredrik

AU - MacLean, Evan L.

AU - Gesquiere, Laurence R.

AU - Leknes, Siri

AU - Lundanes, Elsa

AU - Wilson, Steven Ray

PY - 2016/8/16

Y1 - 2016/8/16

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Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

Abstract

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