Protein kinase C inhibits Na+-K+-2Cl- cotransporter activity in cultured rabbit nonpigmented ciliary epithelium

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We examined the regulation of Na+-K+-2Cl- cotransporter activity by protein kinase C (PKC) in a cell line derived from rabbit nonpigmented ciliary epithelium, Na+-K+-2Cl- cotransporter activity was measured as the rate of bumetanide-sensitive potassium (86Rb) transport. Phorbol 12, 13- dibutyrate (PBDu) was used to activate PKC. PBDu inhibited bumetanide- sensitive potassium (86Rb) uptake, with a half-maximal inhibitory concentration of ~0.1 μM. The inhibitory effect of PBDu on potassium uptake by the Na+-K+-2Cl- cotransporter was abolished by PKC downregulation and diminished by 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, a PKC inhibitor. PBDu inhibited Na+-K+-2Cl- cotransporter-mediated inward potassium (86Rb) transport by ~26% in control cells and by 40% in cells pretreated with ouabain. PKC activation also reduced the rate of bumetanide- sensitive potassium (86Rb) efflux in ouabain-treated cells but not in control (no oubain) cells. PBDu caused little change of intracellular sodium, potassium, or chloride suggesting that an alteration of cytoplasmic ion composition is not responsible for the observed PBDu-induced changes in the rate of either inward or outward potassium movement mediated by the Na+- K+-2Cl- cotransporter.

Original languageEnglish (US)
Pages (from-to)C1553-C1560
JournalAmerican Journal of Physiology - Cell Physiology
Issue number6 36-6
StatePublished - 1994


  • bumetanide
  • ouabain
  • phorbol ester
  • potassium channel
  • sodium-potassium-adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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