Protein "amyloid-like" networks at the phospholipid membrane formed by 4-hydroxy-2-nonenal-modified mitochondrial creatine kinase

Ofelia Maniti, Liberty François-Moutal, Marie France Lecompte, Christian Vial, Michel Lagarde, Michel Guichardant, Olivier Marcillat, Thierry Granjon

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of creatine kinase were previously shown to be affected by 4-HNE. In the present study, we analyzed the effect of 4-HNE on mitochondrial creatine kinase, an abundant protein from the mitochondrial intermembrane space with a key role in mitochondrial physiology. We show that this effect is double: 4-HNE induces a step-wise loss of creatine kinase activity together with a fast protein aggregation. Protein-membrane interaction is affected and amyloid-like networks formed on the biomimetic membrane. These fibrils may disturb mitochondrial organisation both at the membrane and in the inter membrane space.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalMolecular Membrane Biology
Volume32
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • 4-hydroxy-2-nonenal
  • Amyloid-like networks
  • Cardiolipin
  • Mitochondrial creatine kinase
  • Protein aggregation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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