Protease inhibitors of Manduca sexta expressed in transgenic cotton

John C. Thomas, Deanna G. Adams, Veronique D. Keppenne, Catherine C. Wasmann, Judith K. Brown, Michael R. Kanost, Hans J. Bohnert

Research output: Contribution to journalArticlepeer-review

79 Scopus citations


To explore the effectiveness of insect derived protease inhibitors in protecting plants against insect feeding, anti-trypsin, anti-chymotrypsin and anti-elastase protease inhibitor (PI) genes from Manduca sexta L. were expressed in transgenic cotton (Gossypium hirsutum L.). From 198 independent transformants, 35 elite lines were further analyzed. Under the control of the 35S promoter of CaMV, PI accumulated to approximately 0.1% of total protein, depending on the tissue analyzed. Using cell-flow cytometry, DNA content/ nuclei of transgenic and non-transformed cotton were identical. On cotton plants expressing PIs, fecundity of Bemisia tabaci (Genn.), the sweetpotato whitefly, was reduced compared to controls. Expression of these protease inhibitors may reduce the developmental rate of B. tabaci and other insects, and provide a strategy for cotton protection.

Original languageEnglish (US)
Pages (from-to)758-762
Number of pages5
JournalPlant Cell Reports
Issue number12
StatePublished - Oct 1995


  • Plant transformation
  • Protease inhibitor

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


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