Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells

J. A. Wegner, R. Martinez-Zaguilan, R. J. Gillies, P. B. Hoyer

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Thapsigargin (TG) and A23187 were used to examine the regulation of cytosolic free calcium (Ca(i)/2+) in ovine large and small luteal cells. Thapsigargin (50 nM) induced a sustained increase of Ca(i)/2+ in fura 2- acetoxymethyl ester (AM)-loaded cells (large = 1.32 ± 0.07-fold, small = 1.45 ± 0.07-fold, P < 0.05). A23187 (1 μM) induced a rapid transient increase of Ca(i)/2+ (large = 1.37 ± 0.07-fold, small = 1.46 ± 0.10- fold, P < 0.05). In large cells, 0.5 μM prostaglandin F(2α) (PGF(2α)) increased Ca(i)/2+ 1.54 ± 0.11-fold. Pretreatment with 50 nM TG abolished the PGF(2α)-induced calcium response. Pretreatment with PGF(2α) attenuated (P < 0.05) the TG-induced Ca(i)/2+ increase. Progesterone secretion was significantly (P < 0.05) inhibited by incubation with 50 nM TG, 1 μM A23187, and 0.5 μM PGF(2α) in large but not small cells. These data suggest that PGF(2α) releases calcium from a TG-sensitive intracellular calcium pool in ovine large luteal cells.

Original languageEnglish (US)
Pages (from-to)E50-E56
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number1 29-1
StatePublished - 1994


  • intracellular calcium
  • luteolysis
  • ovine luteal cells
  • progesterone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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