Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells

J. A. Wegner; R. Martinez-Zaguilan; R. J. Gillies; P. B. Hoyer

Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells

J. A. Wegner
, R. Martinez-Zaguilan
, R. J. Gillies
, P. B. Hoyer

Physiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Thapsigargin (TG) and A23187 were used to examine the regulation of cytosolic free calcium (Ca(i)/²⁺) in ovine large and small luteal cells. Thapsigargin (50 nM) induced a sustained increase of Ca(i)/²⁺ in fura 2- acetoxymethyl ester (AM)-loaded cells (large = 1.32 ± 0.07-fold, small = 1.45 ± 0.07-fold, P < 0.05). A23187 (1 μM) induced a rapid transient increase of Ca(i)/²⁺ (large = 1.37 ± 0.07-fold, small = 1.46 ± 0.10- fold, P < 0.05). In large cells, 0.5 μM prostaglandin F(2α) (PGF(2α)) increased Ca(i)/²⁺ 1.54 ± 0.11-fold. Pretreatment with 50 nM TG abolished the PGF(2α)-induced calcium response. Pretreatment with PGF(2α) attenuated (P < 0.05) the TG-induced Ca(i)/²⁺ increase. Progesterone secretion was significantly (P < 0.05) inhibited by incubation with 50 nM TG, 1 μM A23187, and 0.5 μM PGF(2α) in large but not small cells. These data suggest that PGF(2α) releases calcium from a TG-sensitive intracellular calcium pool in ovine large luteal cells.

Original language	English (US)
Pages (from-to)	E50-E56
Journal	American Journal of Physiology - Endocrinology and Metabolism
Volume	266
Issue number	1 29-1
State	Published - 1994

Keywords

intracellular calcium
luteolysis
ovine luteal cells
progesterone

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells",

abstract = "Thapsigargin (TG) and A23187 were used to examine the regulation of cytosolic free calcium (Ca(i)/2+) in ovine large and small luteal cells. Thapsigargin (50 nM) induced a sustained increase of Ca(i)/2+ in fura 2- acetoxymethyl ester (AM)-loaded cells (large = 1.32 ± 0.07-fold, small = 1.45 ± 0.07-fold, P < 0.05). A23187 (1 μM) induced a rapid transient increase of Ca(i)/2+ (large = 1.37 ± 0.07-fold, small = 1.46 ± 0.10- fold, P < 0.05). In large cells, 0.5 μM prostaglandin F(2α) (PGF(2α)) increased Ca(i)/2+ 1.54 ± 0.11-fold. Pretreatment with 50 nM TG abolished the PGF(2α)-induced calcium response. Pretreatment with PGF(2α) attenuated (P < 0.05) the TG-induced Ca(i)/2+ increase. Progesterone secretion was significantly (P < 0.05) inhibited by incubation with 50 nM TG, 1 μM A23187, and 0.5 μM PGF(2α) in large but not small cells. These data suggest that PGF(2α) releases calcium from a TG-sensitive intracellular calcium pool in ovine large luteal cells.",

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year = "1994",

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T1 - Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells

AU - Wegner, J. A.

AU - Martinez-Zaguilan, R.

AU - Gillies, R. J.

AU - Hoyer, P. B.

PY - 1994

Y1 - 1994

N2 - Thapsigargin (TG) and A23187 were used to examine the regulation of cytosolic free calcium (Ca(i)/2+) in ovine large and small luteal cells. Thapsigargin (50 nM) induced a sustained increase of Ca(i)/2+ in fura 2- acetoxymethyl ester (AM)-loaded cells (large = 1.32 ± 0.07-fold, small = 1.45 ± 0.07-fold, P < 0.05). A23187 (1 μM) induced a rapid transient increase of Ca(i)/2+ (large = 1.37 ± 0.07-fold, small = 1.46 ± 0.10- fold, P < 0.05). In large cells, 0.5 μM prostaglandin F(2α) (PGF(2α)) increased Ca(i)/2+ 1.54 ± 0.11-fold. Pretreatment with 50 nM TG abolished the PGF(2α)-induced calcium response. Pretreatment with PGF(2α) attenuated (P < 0.05) the TG-induced Ca(i)/2+ increase. Progesterone secretion was significantly (P < 0.05) inhibited by incubation with 50 nM TG, 1 μM A23187, and 0.5 μM PGF(2α) in large but not small cells. These data suggest that PGF(2α) releases calcium from a TG-sensitive intracellular calcium pool in ovine large luteal cells.

AB - Thapsigargin (TG) and A23187 were used to examine the regulation of cytosolic free calcium (Ca(i)/2+) in ovine large and small luteal cells. Thapsigargin (50 nM) induced a sustained increase of Ca(i)/2+ in fura 2- acetoxymethyl ester (AM)-loaded cells (large = 1.32 ± 0.07-fold, small = 1.45 ± 0.07-fold, P < 0.05). A23187 (1 μM) induced a rapid transient increase of Ca(i)/2+ (large = 1.37 ± 0.07-fold, small = 1.46 ± 0.10- fold, P < 0.05). In large cells, 0.5 μM prostaglandin F(2α) (PGF(2α)) increased Ca(i)/2+ 1.54 ± 0.11-fold. Pretreatment with 50 nM TG abolished the PGF(2α)-induced calcium response. Pretreatment with PGF(2α) attenuated (P < 0.05) the TG-induced Ca(i)/2+ increase. Progesterone secretion was significantly (P < 0.05) inhibited by incubation with 50 nM TG, 1 μM A23187, and 0.5 μM PGF(2α) in large but not small cells. These data suggest that PGF(2α) releases calcium from a TG-sensitive intracellular calcium pool in ovine large luteal cells.

KW - intracellular calcium

KW - luteolysis

KW - ovine luteal cells

KW - progesterone

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JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

IS - 1 29-1

ER -

Prostaglandin F(2α) releases calcium from a thapsigargin-sensitive pool in ovine large luteal cells

Abstract

Keywords

ASJC Scopus subject areas

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