TY - JOUR
T1 - Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons
AU - Laeng, H.
AU - Harris, D. T.
AU - Schindler, R.
N1 - Funding Information:
This work was supported by the Swiss National Science Foundation. gratitude to Professor H. Cottier for his encouragement and interest.
PY - 1985/5
Y1 - 1985/5
N2 - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.
AB - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.
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U2 - 10.1016/0014-4827(85)90441-0
DO - 10.1016/0014-4827(85)90441-0
M3 - Article
C2 - 3922778
AN - SCOPUS:0021838284
SN - 0014-4827
VL - 158
SP - 170
EP - 176
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -