Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell: Lack of protoporphyrin IX effects

K. E. Gerrish, C. W. Putnam, H. E. Laird

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. These data suggest that PPIX possesses an affinity for the Nb2 cell PBR (Ki=142 nM). We have previously reported that the peripheral benzodiazepine ligands, Ro5-4864 and PK 11195, modulate prolactin- stimulated mitogenesis in the Nb2 cell(1). In contrast, PPIX, a putative endogenous ligand for the PBR had no effect on prolactin- stimulated mitogenesis in the Nb2 cell over the concentration range from 10-15 M to 10-6 M. Taken together these data show that PPIX has an affinity for the Nb2 cell PBR but does not modulate prolactin- stimulated mitogenesis at concentrations which should bind to the Nb2 cell PBR.

Original languageEnglish (US)
Pages (from-to)1647-1653
Number of pages7
JournalLife Sciences
Volume47
Issue number18
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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