Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell: Lack of protoporphyrin IX effects

K. E. Gerrish; C. W. Putnam; H. E. Laird

doi:10.1016/0024-3205(90)90370-7

Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell: Lack of protoporphyrin IX effects

K. E. Gerrish, C. W. Putnam, H. E. Laird

Surgery

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Abstract

Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [³H] Ro5-4864 binding. These data suggest that PPIX possesses an affinity for the Nb2 cell PBR (K_i=142 nM). We have previously reported that the peripheral benzodiazepine ligands, Ro5-4864 and PK 11195, modulate prolactin- stimulated mitogenesis in the Nb2 cell(1). In contrast, PPIX, a putative endogenous ligand for the PBR had no effect on prolactin- stimulated mitogenesis in the Nb2 cell over the concentration range from 10^-15 M to 10^-6 M. Taken together these data show that PPIX has an affinity for the Nb2 cell PBR but does not modulate prolactin- stimulated mitogenesis at concentrations which should bind to the Nb2 cell PBR.

Original language	English (US)
Pages (from-to)	1647-1653
Number of pages	7
Journal	Life Sciences
Volume	47
Issue number	18
DOIs	https://doi.org/10.1016/0024-3205(90)90370-7
State	Published - 1990

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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@article{c8c6f8d55df24ae08fb4d57459868248,

title = "Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell: Lack of protoporphyrin IX effects",

abstract = "Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. These data suggest that PPIX possesses an affinity for the Nb2 cell PBR (Ki=142 nM). We have previously reported that the peripheral benzodiazepine ligands, Ro5-4864 and PK 11195, modulate prolactin- stimulated mitogenesis in the Nb2 cell(1). In contrast, PPIX, a putative endogenous ligand for the PBR had no effect on prolactin- stimulated mitogenesis in the Nb2 cell over the concentration range from 10-15 M to 10-6 M. Taken together these data show that PPIX has an affinity for the Nb2 cell PBR but does not modulate prolactin- stimulated mitogenesis at concentrations which should bind to the Nb2 cell PBR.",

author = "Gerrish, {K. E.} and Putnam, {C. W.} and Laird, {H. E.}",

note = "Funding Information: This work was supported in part by a grant from the Arizona Disease Control Research Commission (#82-9290 & 82-1685) (H.E.L.), College of Pharmacy Biomedical Research Support Grant (H.E.L.) and a Veterans Administration Merit Award (C.W.P.). The authors are indebted to Dora Chen for technical assistance, Tony Parola for helpful discussions, and Charleen Prytula for manuscript preparation.",

year = "1990",

doi = "10.1016/0024-3205(90)90370-7",

language = "English (US)",

volume = "47",

pages = "1647--1653",

journal = "Life Sciences",

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T1 - Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell

T2 - Lack of protoporphyrin IX effects

AU - Gerrish, K. E.

AU - Putnam, C. W.

AU - Laird, H. E.

N1 - Funding Information: This work was supported in part by a grant from the Arizona Disease Control Research Commission (#82-9290 & 82-1685) (H.E.L.), College of Pharmacy Biomedical Research Support Grant (H.E.L.) and a Veterans Administration Merit Award (C.W.P.). The authors are indebted to Dora Chen for technical assistance, Tony Parola for helpful discussions, and Charleen Prytula for manuscript preparation.

PY - 1990

Y1 - 1990

N2 - Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. These data suggest that PPIX possesses an affinity for the Nb2 cell PBR (Ki=142 nM). We have previously reported that the peripheral benzodiazepine ligands, Ro5-4864 and PK 11195, modulate prolactin- stimulated mitogenesis in the Nb2 cell(1). In contrast, PPIX, a putative endogenous ligand for the PBR had no effect on prolactin- stimulated mitogenesis in the Nb2 cell over the concentration range from 10-15 M to 10-6 M. Taken together these data show that PPIX has an affinity for the Nb2 cell PBR but does not modulate prolactin- stimulated mitogenesis at concentrations which should bind to the Nb2 cell PBR.

AB - Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. These data suggest that PPIX possesses an affinity for the Nb2 cell PBR (Ki=142 nM). We have previously reported that the peripheral benzodiazepine ligands, Ro5-4864 and PK 11195, modulate prolactin- stimulated mitogenesis in the Nb2 cell(1). In contrast, PPIX, a putative endogenous ligand for the PBR had no effect on prolactin- stimulated mitogenesis in the Nb2 cell over the concentration range from 10-15 M to 10-6 M. Taken together these data show that PPIX has an affinity for the Nb2 cell PBR but does not modulate prolactin- stimulated mitogenesis at concentrations which should bind to the Nb2 cell PBR.

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Prolactin-stimulated mitogenesis in the Nb2 rat lymphoma cell: Lack of protoporphyrin IX effects

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