TY - JOUR
T1 - Production of interferon-β upon induction with polyinosinic acid:polycytidylic acid during the cell cycle of human fibroblasts
AU - Tang, Kathy F.J.
AU - Ts'o, Paul O.P.
AU - Lesko, Stephen A.
PY - 1989/4
Y1 - 1989/4
N2 - The production of interferon-β was examined at various stages of the cell cycle in synchronized and unsynchronized cell populations induced by poly(I):poly(C). Human fibroblasts were synchronized with mitotic detachment and, at different stages of the cell cycle, poly(I):poly(C) was added for induction of interferon-β. One hour after induction, cell-free medium was collected and assayed for secreted interferon-β. The cells were then fixed and stained with a DNA-specific fluorochrome, 4′,6-diamidino-2-phenylindole (DAPI), for cell cycle analysis by microfluorometry. The data indicated that interferon-β was produced in every stage examined of the cell cycle. In addition, the level of intracellular interferon-β was quantitatively measured in single cells of an unsynchronized cell population using a specific antibody. In the same individual cell, DAPI fluorescence intensity was measured for determination of the cell cycle position. The results show that interferon-β protein can be detected throughout the cell cycle.
AB - The production of interferon-β was examined at various stages of the cell cycle in synchronized and unsynchronized cell populations induced by poly(I):poly(C). Human fibroblasts were synchronized with mitotic detachment and, at different stages of the cell cycle, poly(I):poly(C) was added for induction of interferon-β. One hour after induction, cell-free medium was collected and assayed for secreted interferon-β. The cells were then fixed and stained with a DNA-specific fluorochrome, 4′,6-diamidino-2-phenylindole (DAPI), for cell cycle analysis by microfluorometry. The data indicated that interferon-β was produced in every stage examined of the cell cycle. In addition, the level of intracellular interferon-β was quantitatively measured in single cells of an unsynchronized cell population using a specific antibody. In the same individual cell, DAPI fluorescence intensity was measured for determination of the cell cycle position. The results show that interferon-β protein can be detected throughout the cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=0024507958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024507958&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(89)90100-6
DO - 10.1016/0014-4827(89)90100-6
M3 - Article
C2 - 2647497
AN - SCOPUS:0024507958
SN - 0014-4827
VL - 181
SP - 432
EP - 441
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -