Production of DNA single strand breaks in rabbit renal tissue after exposure to 1,2-dichlorovinylcysteine

Deborah R. Jaffe; Christopher D. Hassall; A. Jay Gandolfi; Klaus Brendel

doi:10.1016/0300-483X(85)90129-5

Production of DNA single strand breaks in rabbit renal tissue after exposure to 1,2-dichlorovinylcysteine

Deborah R. Jaffe, Christopher D. Hassall, A. Jay Gandolfi, Klaus Brendel

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

S-(trans-1,2-dichlorovinyl)-L-cysteine (DCVC) is a recognized nephrotoxin. To investigate the genotoxic effects of DCVC on the kidney, DNA strand breaks were measured as an indicator of DCVC induced damage. To ascertain if bioactivation of DCVC occured in the kidney, 3 experimental systems were used: in vivo; perfused kidneys; an isolated proximal tubules of albino male rabbitsl. A dose-dependent increase in strand breaks in the kidney tubular DNA occured after in vivo dosing with 5-100 mg/kg DCVC and after in vitro exposure to 10^-5-10^-2 M DCVC. These results demonstrate the genotoxic effect of this compound on renal tissue.

Original language	English (US)
Pages (from-to)	25-33
Number of pages	9
Journal	Toxicology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/0300-483X(85)90129-5
State	Published - Apr 1985

Keywords

1,2-Dichlorovinylcysteine
DNA
Renal
Single-strand breaks

ASJC Scopus subject areas

Toxicology

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10.1016/0300-483X(85)90129-5

Cite this

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title = "Production of DNA single strand breaks in rabbit renal tissue after exposure to 1,2-dichlorovinylcysteine",

abstract = "S-(trans-1,2-dichlorovinyl)-L-cysteine (DCVC) is a recognized nephrotoxin. To investigate the genotoxic effects of DCVC on the kidney, DNA strand breaks were measured as an indicator of DCVC induced damage. To ascertain if bioactivation of DCVC occured in the kidney, 3 experimental systems were used: in vivo; perfused kidneys; an isolated proximal tubules of albino male rabbitsl. A dose-dependent increase in strand breaks in the kidney tubular DNA occured after in vivo dosing with 5-100 mg/kg DCVC and after in vitro exposure to 10-5-10-2 M DCVC. These results demonstrate the genotoxic effect of this compound on renal tissue.",

keywords = "1,2-Dichlorovinylcysteine, DNA, Renal, Single-strand breaks",

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T1 - Production of DNA single strand breaks in rabbit renal tissue after exposure to 1,2-dichlorovinylcysteine

AU - Jaffe, Deborah R.

AU - Hassall, Christopher D.

AU - Jay Gandolfi, A.

AU - Brendel, Klaus

PY - 1985/4

Y1 - 1985/4

N2 - S-(trans-1,2-dichlorovinyl)-L-cysteine (DCVC) is a recognized nephrotoxin. To investigate the genotoxic effects of DCVC on the kidney, DNA strand breaks were measured as an indicator of DCVC induced damage. To ascertain if bioactivation of DCVC occured in the kidney, 3 experimental systems were used: in vivo; perfused kidneys; an isolated proximal tubules of albino male rabbitsl. A dose-dependent increase in strand breaks in the kidney tubular DNA occured after in vivo dosing with 5-100 mg/kg DCVC and after in vitro exposure to 10-5-10-2 M DCVC. These results demonstrate the genotoxic effect of this compound on renal tissue.

AB - S-(trans-1,2-dichlorovinyl)-L-cysteine (DCVC) is a recognized nephrotoxin. To investigate the genotoxic effects of DCVC on the kidney, DNA strand breaks were measured as an indicator of DCVC induced damage. To ascertain if bioactivation of DCVC occured in the kidney, 3 experimental systems were used: in vivo; perfused kidneys; an isolated proximal tubules of albino male rabbitsl. A dose-dependent increase in strand breaks in the kidney tubular DNA occured after in vivo dosing with 5-100 mg/kg DCVC and after in vitro exposure to 10-5-10-2 M DCVC. These results demonstrate the genotoxic effect of this compound on renal tissue.

KW - 1,2-Dichlorovinylcysteine

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KW - Renal

KW - Single-strand breaks

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Production of DNA single strand breaks in rabbit renal tissue after exposure to 1,2-dichlorovinylcysteine

Abstract

Keywords

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