Probing compartment-specific sphingolipids with targeted bacterial sphingomyelinases and ceramidases

Wataru Sakamoto, Daniel Canals, Silvia Salamone, Janet Allopenna, Christopher J. Clarke, Justin Snider, Lina M. Obeid, Yusuf A. Hannun

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Sphingolipids contribute to the regulation of cell and tissue homeostasis, and disorders of sphingolipid metabolism lead to diseases such as inflammation, stroke, diabetes, and cancer. Sphingolipid metabolic pathways involve an array of enzymes that reside in specific subcellular organelles, resulting in the formation of many diverse sphingolipids with distinct molecular species based on the diversity of the ceramide (Cer) structure. In order to probe compartment-specific metabolism of sphingolipids in this study, we analyzed the Cer and SM species preferentially produced in the inner plasma membrane (PM), Golgi apparatus, ER, mitochondria, nucleus, and cytoplasm by using compartmentally targeted bacterial SMases and ceramidases. The results showed that the length of the acyl chain of Cer becomes longer according to the progress of Cer from synthesis in the ER to the Golgi apparatus, then to the PM. These findings suggest that each organelle shows different properties of SM-derived Cers consistent with its emerging distinct functions in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)1841-1850
Number of pages10
JournalJournal of Lipid Research
Volume60
Issue number11
DOIs
StatePublished - 2019
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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