Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Woo Ho-Hyung; Lindy A. Brigham; Martha C. Hawes

doi:10.1016/0378-1119(94)90715-3

Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Woo Ho-Hyung
, Lindy A. Brigham
, Martha C. Hawes

Environmental Science-Res

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys⁸⁵) found in UBCs so far described is also conserved in the P. sativum sequence.

Original language	English (US)
Pages (from-to)	369-370
Number of pages	2
Journal	Gene
Volume	148
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90715-3
State	Published - Oct 21 1994

Keywords

Amino-acid sequence conservation
pea
plants

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90715-3

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@article{0c5045f813044608af8a10ed3866f147,

title = "Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum",

abstract = "The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97\% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80\% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.",

keywords = "Amino-acid sequence conservation, pea, plants",

author = "Woo Ho-Hyung and Brigham, \{Lindy A.\} and Hawes, \{Martha C.\}",

note = "Funding Information: This work was supported by USDA (92-37303-7795) and DOE (DE-FG02-91ER20044) to M.C.H.",

year = "1994",

month = oct,

day = "21",

doi = "10.1016/0378-1119(94)90715-3",

language = "English (US)",

volume = "148",

pages = "369--370",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

AU - Ho-Hyung, Woo

AU - Brigham, Lindy A.

AU - Hawes, Martha C.

N1 - Funding Information: This work was supported by USDA (92-37303-7795) and DOE (DE-FG02-91ER20044) to M.C.H.

PY - 1994/10/21

Y1 - 1994/10/21

N2 - The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.

AB - The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.

KW - Amino-acid sequence conservation

KW - pea

KW - plants

UR - https://www.scopus.com/pages/publications/0028004307

UR - https://www.scopus.com/pages/publications/0028004307#tab=citedBy

U2 - 10.1016/0378-1119(94)90715-3

DO - 10.1016/0378-1119(94)90715-3

M3 - Article

C2 - 7958971

AN - SCOPUS:0028004307

SN - 0378-1119

VL - 148

SP - 369

EP - 370

JO - Gene

JF - Gene

IS - 2

ER -

Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Abstract

Keywords

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