Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Woo Ho-Hyung; Lindy A. Brigham; Martha C. Hawes

doi:10.1016/0378-1119(94)90715-3

Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Woo Ho-Hyung, Lindy A. Brigham, Martha C. Hawes

Environmental Science, Research

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Abstract

The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys⁸⁵) found in UBCs so far described is also conserved in the P. sativum sequence.

Original language	English (US)
Pages (from-to)	369-370
Number of pages	2
Journal	Gene
Volume	148
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90715-3
State	Published - Oct 21 1994

Keywords

Amino-acid sequence conservation
pea
plants

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90715-3

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title = "Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum",

abstract = "The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.",

keywords = "Amino-acid sequence conservation, pea, plants",

author = "Woo Ho-Hyung and Brigham, {Lindy A.} and Hawes, {Martha C.}",

note = "Funding Information: This work was supported by USDA (92-37303-7795) and DOE (DE-FG02-91ER20044) to M.C.H.",

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T1 - Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

AU - Ho-Hyung, Woo

AU - Brigham, Lindy A.

AU - Hawes, Martha C.

N1 - Funding Information: This work was supported by USDA (92-37303-7795) and DOE (DE-FG02-91ER20044) to M.C.H.

PY - 1994/10/21

Y1 - 1994/10/21

N2 - The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.

AB - The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.

KW - Amino-acid sequence conservation

KW - pea

KW - plants

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JO - Gene

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Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Abstract

Keywords

ASJC Scopus subject areas

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