Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes

Kenneth Ramos; Daniel Acosta

doi:10.1016/0300-483X(83)90059-8

Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes

Kenneth Ramos, Daniel Acosta

Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Primary cultures of rat myocytes were exposed to various doses of L-isoproterenol (ISO) for 4, 12 and 24 h. L-ascorbic acid (AA) was added to some cultures immediatedly after exposure to ISO to determine if antioxidants reduce the toxicity caused by ISO. Leakage of lactate dehydrogenase (LDH) and cell viability were used as indices of cell injury. A significant increase in LDH release was found 24 h after exposure to 1 × 10^-4 M ISO alone. Higher doses (5 × 10^-4 and 1 × 10^-3 M) caused significant enzyme release 4, 12 and 24 h after exposure. The viability of cultures exposed to toxic doses of ISO for 4 h was not affected. A dose-dependent decrease in cell viability was observed 12 h and 24 h after exposure to ISO. L-ascorbic acid (5 × 10^-3 and 1.5 × 10^-2 M) significantly reduced the LDH release caused by ISO. The enzyme release from cultures exposed to 5 × 10^-4 and 5 × 10^-3 M AA alone was similar to that of control cultures. Howecer, 1.5 × 10^-2 and 3 × 10^-2 M AA caused marked LDH release. The viability of cultures subjected to ISo in the presence of 5 × 10^-3 M AA was similar to that of controls. Our results show that AA protects against the LDH release and decreased cellular viability cuased by toxic doses of ISO.

Original language	English (US)
Pages (from-to)	81-90
Number of pages	10
Journal	Toxicology
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/0300-483X(83)90059-8
State	Published - Jan 1983

Keywords

Ascorbic acid
Cardiotoxicity
LDH release
Myocyte culturesl Isoproterenol

ASJC Scopus subject areas

Toxicology

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10.1016/0300-483X(83)90059-8

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@article{f0375b6f63df457dbb5dab0d2aff700d,

title = "Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes",

abstract = "Primary cultures of rat myocytes were exposed to various doses of L-isoproterenol (ISO) for 4, 12 and 24 h. L-ascorbic acid (AA) was added to some cultures immediatedly after exposure to ISO to determine if antioxidants reduce the toxicity caused by ISO. Leakage of lactate dehydrogenase (LDH) and cell viability were used as indices of cell injury. A significant increase in LDH release was found 24 h after exposure to 1 × 10-4 M ISO alone. Higher doses (5 × 10-4 and 1 × 10-3 M) caused significant enzyme release 4, 12 and 24 h after exposure. The viability of cultures exposed to toxic doses of ISO for 4 h was not affected. A dose-dependent decrease in cell viability was observed 12 h and 24 h after exposure to ISO. L-ascorbic acid (5 × 10-3 and 1.5 × 10-2 M) significantly reduced the LDH release caused by ISO. The enzyme release from cultures exposed to 5 × 10-4 and 5 × 10-3 M AA alone was similar to that of control cultures. Howecer, 1.5 × 10-2 and 3 × 10-2 M AA caused marked LDH release. The viability of cultures subjected to ISo in the presence of 5 × 10-3 M AA was similar to that of controls. Our results show that AA protects against the LDH release and decreased cellular viability cuased by toxic doses of ISO.",

keywords = "Ascorbic acid, Cardiotoxicity, LDH release, Myocyte culturesl Isoproterenol",

author = "Kenneth Ramos and Daniel Acosta",

note = "Funding Information: The technical assistance of Christine Grassl and Dawn Walsh is gratefuly appreciated. This project was supported in part by BRSG S07RR05849 awarded by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health and by a grant from the University of Texas Research Institute.",

year = "1983",

month = jan,

doi = "10.1016/0300-483X(83)90059-8",

language = "English (US)",

volume = "26",

pages = "81--90",

journal = "Toxicology",

issn = "0300-483X",

publisher = "Elsevier Ireland Ltd",

number = "1",

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T1 - Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes

AU - Ramos, Kenneth

AU - Acosta, Daniel

N1 - Funding Information: The technical assistance of Christine Grassl and Dawn Walsh is gratefuly appreciated. This project was supported in part by BRSG S07RR05849 awarded by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health and by a grant from the University of Texas Research Institute.

PY - 1983/1

Y1 - 1983/1

N2 - Primary cultures of rat myocytes were exposed to various doses of L-isoproterenol (ISO) for 4, 12 and 24 h. L-ascorbic acid (AA) was added to some cultures immediatedly after exposure to ISO to determine if antioxidants reduce the toxicity caused by ISO. Leakage of lactate dehydrogenase (LDH) and cell viability were used as indices of cell injury. A significant increase in LDH release was found 24 h after exposure to 1 × 10-4 M ISO alone. Higher doses (5 × 10-4 and 1 × 10-3 M) caused significant enzyme release 4, 12 and 24 h after exposure. The viability of cultures exposed to toxic doses of ISO for 4 h was not affected. A dose-dependent decrease in cell viability was observed 12 h and 24 h after exposure to ISO. L-ascorbic acid (5 × 10-3 and 1.5 × 10-2 M) significantly reduced the LDH release caused by ISO. The enzyme release from cultures exposed to 5 × 10-4 and 5 × 10-3 M AA alone was similar to that of control cultures. Howecer, 1.5 × 10-2 and 3 × 10-2 M AA caused marked LDH release. The viability of cultures subjected to ISo in the presence of 5 × 10-3 M AA was similar to that of controls. Our results show that AA protects against the LDH release and decreased cellular viability cuased by toxic doses of ISO.

AB - Primary cultures of rat myocytes were exposed to various doses of L-isoproterenol (ISO) for 4, 12 and 24 h. L-ascorbic acid (AA) was added to some cultures immediatedly after exposure to ISO to determine if antioxidants reduce the toxicity caused by ISO. Leakage of lactate dehydrogenase (LDH) and cell viability were used as indices of cell injury. A significant increase in LDH release was found 24 h after exposure to 1 × 10-4 M ISO alone. Higher doses (5 × 10-4 and 1 × 10-3 M) caused significant enzyme release 4, 12 and 24 h after exposure. The viability of cultures exposed to toxic doses of ISO for 4 h was not affected. A dose-dependent decrease in cell viability was observed 12 h and 24 h after exposure to ISO. L-ascorbic acid (5 × 10-3 and 1.5 × 10-2 M) significantly reduced the LDH release caused by ISO. The enzyme release from cultures exposed to 5 × 10-4 and 5 × 10-3 M AA alone was similar to that of control cultures. Howecer, 1.5 × 10-2 and 3 × 10-2 M AA caused marked LDH release. The viability of cultures subjected to ISo in the presence of 5 × 10-3 M AA was similar to that of controls. Our results show that AA protects against the LDH release and decreased cellular viability cuased by toxic doses of ISO.

KW - Ascorbic acid

KW - Cardiotoxicity

KW - LDH release

KW - Myocyte culturesl Isoproterenol

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SN - 0300-483X

VL - 26

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JO - Toxicology

JF - Toxicology

IS - 1

ER -

Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes

Abstract

Keywords

ASJC Scopus subject areas

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