@article{0726ffbd36f04b68a971a71b15011eb1,
title = "Presynaptic regulation of neurotransmission in Drosophila by the G protein-coupled receptor Methuselah",
abstract = "Regulation of synaptic strength is essential for neuronal information processing, but the molecular mechanisms that control changes in neuroexocytosis are only partially known. Here we show that the putative G protein-coupled receptor Methuselah (Mth) is required in the presynaptic motor neuron to acutely upregulate neurotransmitter exocytosis at larval Drosophila NMJs. Mutations in the mth gene reduce evoked neurotransmitter release by ∼50%, and decrease synaptic area and the density of docked and clustered vesicles. Pre- but not postsynaptic expression of normal Mth restored normal release in mth mutants. Conditional expression of Mth restored normal release and normal vesicle docking and clustering but not the reduced size of synaptic sites, suggesting that Mth acutely adjusts vesicle trafficking to synaptic sites.",
author = "Wei Song and Ravi Ranjan and Ken Dawson-Scully and Peter Bronk and Leo Marin and Laurent Seroude and Lin, {Yi Jyun} and Zhiping Nie and Atwood, {Harold L.} and Seymour Benzer and Zinsmaier, {Konrad E.}",
note = "Funding Information: We thank Dr. Corey Goodman for Fas-II antibodies and the CD8-GFP-Sh strain and Drs. Alexander Petrenko and Yuri Ushkaryov for purified latro- and latroinsectotoxins. We thank Sheng Wang for assistance with electron microscopy. We thank Drs. Peter Sterling, Mike Nusbaum, Brian Salzberg, and Phil Haydon for critical comments on the manuscript and Dr. Irwin Levitan for his extraordinary support during the last stages of this study. This work has been in part supported by grants to K.E.Z. from the National Science Foundation (IBN-9604889) and the National Institutes of Health (RO1NS38274), by a National Research Service Award to P.B. from NIMH (MH12611F31), by a Provost Award to W.S., by grants to S.B. from the National Science Foundation (MCB9907939), the National Institutes of Health (AG16630 and EY09278), and the Ellison Foundation, and grants to H.L.A. from the Natural Sciences and Engineering Research Council of Canada (NSERC).",
year = "2002",
month = sep,
day = "26",
doi = "10.1016/S0896-6273(02)00932-7",
language = "English (US)",
volume = "36",
pages = "105--119",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}