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Preparation of biomolecule gel matrices for electron microscopy

  • Sonal Padalkar
  • , Jianxiu Zhao
  • , Kate Stuart
  • , Alyssa Panitch
  • , Jenna Rickus
  • , Lia Stanciu

Research output: Contribution to journalArticlepeer-review

Abstract

We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule-gel matrix systems.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalUltramicroscopy
Volume108
Issue number4
DOIs
StatePublished - Mar 2008
Externally publishedYes

Keywords

  • Biomolecules
  • Electron microscopy
  • Gels

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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