Preparation and cytotoxicity comparison of type a gelatin nanoparticles with recombinant human gelatin nanoparticles

Young Wook Won, Yong Hee Kim

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Gelatin nanoparticles derived from bovine or porcine have been developed as various types of drug delivery system, and they need to be cross-linked to maintain their physicochemical properties in aqueous environments. Although gelatin is a widely used material in pharmaceutical industries, the safety issue of animal-origin gelatins, such as transmissible mad cow disease and anaphylaxis, remains to be solved. The purpose of this study was to prepare type A gelatin (GA) nanoparticles by modified, two-step, desolvation method and compare the toxicity of the resulting GA nanoparticles with recombinant human gelatin (rHG) nanoparticles. The GA nanoparticles were characterized, and drug loading and release pattern were measured. FITC-BSA, a model protein, was efficiently loaded in the nanoparticles and then released in a biphasic and sustained release pattern without an initial burst. In particular, the cell viability of the GA nanoparticles was less than that of the rHG nanoparticles. This finding suggests that rHG nanoparticles should be considered as an alternative to animal-origin gelatin nanoparticles in order to minimize the safety problems.

Original languageEnglish (US)
Pages (from-to)464-468
Number of pages5
JournalMacromolecular Research
Volume17
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

Keywords

  • Drug delivery.
  • Gelatin
  • Nanoparticles
  • Protein drug
  • Recombinant human gelatin

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Preparation and cytotoxicity comparison of type a gelatin nanoparticles with recombinant human gelatin nanoparticles'. Together they form a unique fingerprint.

Cite this