Preliminary evidence of free radical formation during argon fluoride excimer laser irradiation of corneal tissue

R. J. Landry; G. H. Pettit; D. W. Hahn; M. N. Ediger; G. C. Yang

Preliminary evidence of free radical formation during argon fluoride excimer laser irradiation of corneal tissue

R. J. Landry, G. H. Pettit, D. W. Hahn, M. N. Ediger, G. C. Yang

Research output: Contribution to journal › Article › peer-review

Abstract

Electron paramagnetic resonance (EPR) spectroscopy has been used to observe transient free radicals produced in corneal tissue by 193 nm radiation from an argon fluoride excimer laser in vitro. Six bovine tissue strips measuring ~2.0 cm x 0.2 cm were immersed in liquid nitrogen and each irradiated with 4.5 mJ laser pulses at a repetition rate of 10 Hz for one minute (pulse fluence ~90 mJ/cm²). Five identical untreated samples served as controls. Subsequent EPR spectroscopy at -150°C showed a pronounced free-radical signal in each irradiated sample, but in none of the controls. This preliminary evidence of radical formation should prove useful in better understanding the laser/tissue interaction.

Original language	English (US)
Pages (from-to)	87-90
Number of pages	4
Journal	Lasers and Light in Ophthalmology
Volume	6
Issue number	2
State	Published - 1994
Externally published	Yes

Keywords

electron paramagnetic resonance
photorefractive keratectomy

ASJC Scopus subject areas

Ophthalmology

Cite this

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title = "Preliminary evidence of free radical formation during argon fluoride excimer laser irradiation of corneal tissue",

abstract = "Electron paramagnetic resonance (EPR) spectroscopy has been used to observe transient free radicals produced in corneal tissue by 193 nm radiation from an argon fluoride excimer laser in vitro. Six bovine tissue strips measuring ~2.0 cm x 0.2 cm were immersed in liquid nitrogen and each irradiated with 4.5 mJ laser pulses at a repetition rate of 10 Hz for one minute (pulse fluence ~90 mJ/cm2). Five identical untreated samples served as controls. Subsequent EPR spectroscopy at -150°C showed a pronounced free-radical signal in each irradiated sample, but in none of the controls. This preliminary evidence of radical formation should prove useful in better understanding the laser/tissue interaction.",

keywords = "electron paramagnetic resonance, photorefractive keratectomy",

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year = "1994",

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T1 - Preliminary evidence of free radical formation during argon fluoride excimer laser irradiation of corneal tissue

AU - Landry, R. J.

AU - Pettit, G. H.

AU - Hahn, D. W.

AU - Ediger, M. N.

AU - Yang, G. C.

PY - 1994

Y1 - 1994

N2 - Electron paramagnetic resonance (EPR) spectroscopy has been used to observe transient free radicals produced in corneal tissue by 193 nm radiation from an argon fluoride excimer laser in vitro. Six bovine tissue strips measuring ~2.0 cm x 0.2 cm were immersed in liquid nitrogen and each irradiated with 4.5 mJ laser pulses at a repetition rate of 10 Hz for one minute (pulse fluence ~90 mJ/cm2). Five identical untreated samples served as controls. Subsequent EPR spectroscopy at -150°C showed a pronounced free-radical signal in each irradiated sample, but in none of the controls. This preliminary evidence of radical formation should prove useful in better understanding the laser/tissue interaction.

AB - Electron paramagnetic resonance (EPR) spectroscopy has been used to observe transient free radicals produced in corneal tissue by 193 nm radiation from an argon fluoride excimer laser in vitro. Six bovine tissue strips measuring ~2.0 cm x 0.2 cm were immersed in liquid nitrogen and each irradiated with 4.5 mJ laser pulses at a repetition rate of 10 Hz for one minute (pulse fluence ~90 mJ/cm2). Five identical untreated samples served as controls. Subsequent EPR spectroscopy at -150°C showed a pronounced free-radical signal in each irradiated sample, but in none of the controls. This preliminary evidence of radical formation should prove useful in better understanding the laser/tissue interaction.

KW - electron paramagnetic resonance

KW - photorefractive keratectomy

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Preliminary evidence of free radical formation during argon fluoride excimer laser irradiation of corneal tissue

Abstract

Keywords

ASJC Scopus subject areas

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