Electron paramagnetic resonance (EPR) spectroscopy has been used to observe transient free radicals produced in corneal tissue by 193 nm radiation from an argon fluoride excimer laser in vitro. Six bovine tissue strips measuring ~2.0 cm x 0.2 cm were immersed in liquid nitrogen and each irradiated with 4.5 mJ laser pulses at a repetition rate of 10 Hz for one minute (pulse fluence ~90 mJ/cm2). Five identical untreated samples served as controls. Subsequent EPR spectroscopy at -150°C showed a pronounced free-radical signal in each irradiated sample, but in none of the controls. This preliminary evidence of radical formation should prove useful in better understanding the laser/tissue interaction.
|Original language||English (US)|
|Number of pages||4|
|Journal||Lasers and Light in Ophthalmology|
|State||Published - 1994|
- electron paramagnetic resonance
- photorefractive keratectomy
ASJC Scopus subject areas