TY - JOUR
T1 - Prediction of itching diagnostic marker through RNA sequencing of contact hypersensitivity and skin scratching stimulation mice models
AU - Kim, Young Won
AU - Zhou, Tong
AU - Ko, Eun A.
AU - Kim, Seongtae
AU - Lee, Donghee
AU - Seo, Yelim
AU - Kwon, Nahee
AU - Choi, Taeyeon
AU - Lim, Heejung
AU - Cho, Sungvin
AU - Bae, Gwanhui
AU - Hwang, Yuseong
AU - Kim, Dojin
AU - Park, Hyewon
AU - Lee, Minjae
AU - Jang, Eunkyung
AU - Choi, Jeongyoon
AU - Bae, Hyemi
AU - Lim, Inja
AU - Bang, Hyoweon
AU - Ko, Jae Hong
N1 - Funding Information:
This research was supported by Basic Science Research Pro-
Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03932890). This research was supported by the Chung-Ang University Research Grants in 2018.
Publisher Copyright:
Copyright © Korean J Physiol Pharmacol.
PY - 2019/3
Y1 - 2019/3
N2 - Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a, Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.
AB - Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a, Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.
KW - Cytokines
KW - Pruritus
KW - RNA sequence analysis
KW - Transient receptor potential channels
KW - Wound healing
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U2 - 10.4196/kjpp.2019.23.2.151
DO - 10.4196/kjpp.2019.23.2.151
M3 - Article
AN - SCOPUS:85064417019
SN - 1226-4512
VL - 23
SP - 151
EP - 159
JO - Korean Journal of Physiology and Pharmacology
JF - Korean Journal of Physiology and Pharmacology
IS - 2
ER -