TY - JOUR
T1 - Practical considerations for preparing polymerized phospholipid bilayer capillary coatings for protein separations
AU - Adem, Seid M.
AU - Mansfield, Elisabeth
AU - Keogh, John P.
AU - Hall, Henry K.
AU - Aspinwall, Craig A.
N1 - Funding Information:
This study was supported by the National Institutes of Health under grant numbers GM095763 and EB007047 .
PY - 2013/4/15
Y1 - 2013/4/15
N2 - Phosphorylcholine (PC) based phospholipid bilayers have proven useful as capillary coating materials due to their inherent resistance to non-specific protein adsorption. The primary limitation of this important class of capillary coatings remains the limited long-term chemical and physical stability of the coatings. Recently, a method for increasing phospholipid coating stability in fused silica capillaries via utilization of polymerized, synthetic phospholipids was reported. Here, we expand upon these studies by investigating polymerized lipid bilayer capillary coatings with respect to separation performance including run-to-run, day-to-day and column-to-column reproducibility and long-term stability. In addition, the effects of pH and capillary inner diameter on polymerized phospholipid coated capillaries were investigated to identify optimized coating conditions. The coatings are stabilized for protein separations across a wide range of pH values (4.0-9.3), a unique property for capillary coating materials. Additionally, smaller inner diameter capillaries (≤50. μm) were found to yield marked enhancements in coating stability and reproducibility compared to wider bore capillaries, demonstrating the importance of capillary size for separations employing polymerized phospholipid coatings.
AB - Phosphorylcholine (PC) based phospholipid bilayers have proven useful as capillary coating materials due to their inherent resistance to non-specific protein adsorption. The primary limitation of this important class of capillary coatings remains the limited long-term chemical and physical stability of the coatings. Recently, a method for increasing phospholipid coating stability in fused silica capillaries via utilization of polymerized, synthetic phospholipids was reported. Here, we expand upon these studies by investigating polymerized lipid bilayer capillary coatings with respect to separation performance including run-to-run, day-to-day and column-to-column reproducibility and long-term stability. In addition, the effects of pH and capillary inner diameter on polymerized phospholipid coated capillaries were investigated to identify optimized coating conditions. The coatings are stabilized for protein separations across a wide range of pH values (4.0-9.3), a unique property for capillary coating materials. Additionally, smaller inner diameter capillaries (≤50. μm) were found to yield marked enhancements in coating stability and reproducibility compared to wider bore capillaries, demonstrating the importance of capillary size for separations employing polymerized phospholipid coatings.
KW - Bis-SorbPC
KW - Capillary coating
KW - Phospholipids
KW - Polymerized lipid bilayer
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U2 - 10.1016/j.aca.2013.02.023
DO - 10.1016/j.aca.2013.02.023
M3 - Article
C2 - 23540253
AN - SCOPUS:84875597755
VL - 772
SP - 93
EP - 98
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -