Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein

Jacob C. Schwartz, David R. Corey

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Target validation for small RNAs in cells can be a confusing task wrought with pitfalls and false leads. One technique for validating in vivo targets of small RNAs is immunoprecipitation of target RNAs using antibodies again the RNAi machinery. Antigene RNAs (agRNAs) regulate transcription in human cells using machinery from the RNAi regulatory pathway – namely argonaute proteins. Here we describe a technique for validating targets of agRNAs using RNA immunoprecipitation with antibodies against human argonaute proteins. This technique can be used to detect interactions of argonaute proteins in the cell nucleus with their targets, lowly expressed noncoding RNA transcripts.

Original languageEnglish (US)
Title of host publicationTherapeutic Oligonucleotides
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages15
ISBN (Print)9781617791871
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Noncoding RNA
  • RNA immunoprecipitation
  • antigene RNAs
  • argonaute
  • transcriptional gene silencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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