Potential role of atomic force microscopy in systems biology

Srinivasan Ramachandran, Fernando Teran Arce, Ratnesh Lal

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations

Abstract

Systems biology is a quantitative approach for understanding a biological system at its global level through systematic perturbation and integrated analysis of all its components. Simultaneous acquisition of information data sets pertaining to the system components (e.g., genome, proteome) is essential to implement this approach. There are limitations to such an approach in measuring gene expression levels and accounting for all proteins in the system. The success of genomic studies is critically dependent on polymerase chain reaction (PCR) for its amplification, but PCR is very uneven in amplifying the samples, ineffective in scarce samples and unreliable in low copy number transcripts. On the other hand, lack of amplifying techniques for proteins critically limits their identification to only a small fraction of high concentration proteins. Atomic force microscopy (AFM), AFM cantilever sensors, and AFM force spectroscopy in particular, could address these issues directly. In this article, we reviewed and assessed their potential role in systems biology.

Original languageEnglish (US)
Pages (from-to)702-716
Number of pages15
JournalWiley Interdisciplinary Reviews: Systems Biology and Medicine
Volume3
Issue number6
DOIs
StatePublished - Nov 2011
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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