Resent studies investigating aeroponic cultivation of medicinal plants have provided encouraging results for increasing yields, shortening time to maturity, and improving consistency and overall quality of produce over field production. The goal of the current study was to determine the applicability of aeroponic technology for the cultivation of the traditionally field grown herbaceous medicinal plant Urtica dioica. In addition, we investigated if control of nutrient delivery and repeated harvesting practices could be utilized to increase and direct yield of desired plant parts. Comparison of root and shoot dry weights between treatments revealed; (1) U. dioica cultivated in soil-less medium yielded equal shoot biomass and greater root biomass than aeroponically cultivated plants, (2) potassium and phosphorus ratios within the nutrient solution had no significant impact on yield or biomass allocation, and (3) multiple harvesting of aeroponic roots and shoots yielded greater total biomass of both roots and shoots than a multi-crop replanting strategy. Results suggest aeroponic technology could be a powerful tool for the cultivation U. dioica as well as a variety of other important herbaceous medicinal plants. However, further optimization of the plant growing environment is required to maximize and direct growth.