Poly(Amido Amine)s Containing Agmatine and Butanol Side Chains as Efficient Gene Carriers

Young Wook Won, Marc Ankoné, Johan F.J. Engbersen, Jan Feijen, Sung Wan Kim

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier. Poly cystamine bisacrylamide with 4-aminobutanol and 4-aminobutylguanidine (agmatine) (CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier.

Original languageEnglish (US)
Pages (from-to)619-626
Number of pages8
JournalMacromolecular Bioscience
Volume16
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

Keywords

  • biological applications of polymers
  • drug delivery systems
  • gene delivery
  • nanoparticles
  • structure-property relations
  • transfection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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