Plasma protein denaturation with graded heat exposure

R. Vazquez, D. F. Larson

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


During cardiopulmonary bypass (CPB), perfusion at tepid temperatures (33-35 C) is recommended to avoid high temperature cerebral hyperthermia during and after the operation. However, the ideal temperature for uncomplicated adult cardiac surgery is an unsettled question. Typically, the heat exchanger maximum temperature is monitored between 40-42 C to prevent denaturation of plasma proteins, but studies have not been performed to make these conclusions. Therefore, our hypothesis was to determine the temperature in which blood plasma protein degradation occurs after 2 hours of heat exposure. As a result, blood plasma proteins were exposed to heat in the 37-50 C range for 2 hours. Plasma protein samples were loaded onto an 8-12% gradient gel for SDS-PAGE and low molecular weight plasma protein degradation was detected with graded heat exposure. Protein degradation was first detected between 43-45 C of heat exposure. This study supports the practice of monitoring the heat exchanger between 40-42 C to prevent denaturation of plasma proteins.

Original languageEnglish (US)
Pages (from-to)557-559
Number of pages3
JournalPerfusion (United Kingdom)
Issue number6
StatePublished - Nov 2013


  • degradation
  • denaturation
  • heat exposure
  • plasma protein

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Safety Research
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing


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