Plant species successfully micropropagated photoautotrophically

C. Kubota, F. Afreen, S. M.A. Zobayed

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


The concept of photoautotrophic micropropagation is derived from the research that revealed relatively high photosynthetic ability of chlorophyllous cultures such as leafy explants, cotyledonary stage somatic embryos and plantlets in vitro. While studying the environmental conditions of tissue culture vessels containing leafy green plantlets of Caiathea, Spatihphyllum, Phirodendron imbe Nepenthes, Dracaena, Cymbidium, Limonium, Syngonium, Cordyline, Ficus lyrata, Fujiwara et al. (1987) found that in vitro plantlets could not fully achieve their photosynthetic ability during the light period because the CO2 concentration in the closed vessels were too low in most of the light periods. Therefore, they concluded that the tissue cultured plantlets can be grown photoautotrophically (to be able to photosynthesise) during and after the multiplication stage by improving the CO2 and light environments in the culture vessels. In the following year (1988) several studies were reported in which photoautotrophic micropropagation, that is, growing plantlets in vitro in sugar-free medium was achieved. First of all, Kozai et al. (1988) successfully cultured potato (Solanum tuberosum L.) plantlets in sugar-free medium with a goal to develop an automated mass propagation system for producing diseasefree seed-potato tubers and disease-free potato plantlets. In another study, Strawberry (Fragaria x ananassa) plants have been grown successfully under photoautotrophic conditions by Kozai and Sekimoto (1988). Carnation (Dianthus caryophyllus L.) (Kozai and Iwanami, 1988) and tobacco (Nicotiana tabacum L.) plants (Pospisilova et al., 1988) were also grown photoautotrophically in the same year (1988). The concept of growing plantlets in a large vessel under photoautotrophic conditions with forced ventilation system was first successfully implemented by Fujiwara et al., (1988). The vessel, volume of 19 L (58 cm long, 28 cm wide and 12 cm high) with an attached air pump for the forced ventilation, was used to enhance the photoautotrophic growth of strawberry (Fragaria x ananassa Duch.) explants and/or plants during the rooting and acclimatization stages. This was an aseptic micro-hydroponic system with a nutrient solution control system. Since then the trend of developing protocols for the in vitro growth of plants under photoautotrophic conditions started and is still continuing and so far nearly 50 different plant species have been reported to grow successfully under photoautotrophic conditions. Among these potato plant has been studied most extensively by many authors (Kozai et al., 1988; Takazawa and Kozai, 1992; Kozai et al., 1992 and 1995b; Tanaka et al., 1992; Miyashita et al., 1997; Kitaya et al., 1995c and 1997a; Fujiwara et al., 1995; Hayashi et al., 1995; Miyashita et al., 1995 and 1996; Roche et al., 1996; Niu. and Kozai, 1997; Niu et al., 1997; Zobayed et al., 1999a; Kim et al., 1999; Xiao et al., 2000; Pruski et al., 2002) followed by sweetpotato (Nagatome et al., 2000, Kozai et al., 1996; Ohyama and Kozai, 1997; Niu et al., 1998; Afreen et al., 1999; Zobayed et al., 1999; Afreen et al., 2000; t Zobayed et al., 2000; Heo and Kozai, 1999; Wilson et al., 2000; Kubota et al., 2002).

Original languageEnglish (US)
Title of host publicationPhotoautotrophic (sugar-free medium) Micropropagation as a New Micropropagation and Transplant Production System
PublisherSpringer Netherlands
Number of pages24
ISBN (Print)9781402031250
StatePublished - 2005


  • Forced ventilation
  • Natural ventilation
  • Nutrient medium
  • Photoautotrophic micropropagation
  • Supporting material

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences


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