TY - JOUR
T1 - Plant part selection and evaluation of factors affecting analysis and recovery of nitrate in irrigated durum wheat tissue
AU - Knowles, T. C.
AU - Doerge, T. A.
AU - Ottman, M. J.
PY - 1989/3/1
Y1 - 1989/3/1
N2 - Periodic nitrate tissue tests are used to determine nitrogen (N) fertility status of a variety of crops. Data on the importance of plant part selection, sample handling techniques, grinding criteria and extraction conditions in NO3-N analysis of wheat tissue are essential if the procedure is to achieve widespread adoption and use. Detailed partitioning of field grown durum spring wheat (Triticum durum) plants at the Feekes 2 (3–4 leaf), 6 (joint) and 10 (boot) growth stages was conducted to document which plant part exhibits the greatest accumulation of NO3-N. Sample handling, fineness of tissue grinding, different tissue: extractant ratios and extraction times were examined to determine their effects on NO3-N recovery. Partitioning data confirmed previous findings which identified the basal stem between ground level and the seed prior to jointing and the 5 cm of stem just above ground level thereafter as the plant parts showing the greatest accumulation of NO3-N. Therefore, these plant parts have the greatest potential as indicators of the N nutritional status of durum spring wheat. Optimal recovery of tissue NO3-N was obtained when stem tissue was separated immediately in the field and dried within 8 hours of sampling, ground to pass a 0.55 mm mesh screen, and extracted for at least 30 minutes when using a sample size of 0.1000 g in conjunction with 25 ml of extractant (i.e. 1:250 plant tissue to extractant ratio).
AB - Periodic nitrate tissue tests are used to determine nitrogen (N) fertility status of a variety of crops. Data on the importance of plant part selection, sample handling techniques, grinding criteria and extraction conditions in NO3-N analysis of wheat tissue are essential if the procedure is to achieve widespread adoption and use. Detailed partitioning of field grown durum spring wheat (Triticum durum) plants at the Feekes 2 (3–4 leaf), 6 (joint) and 10 (boot) growth stages was conducted to document which plant part exhibits the greatest accumulation of NO3-N. Sample handling, fineness of tissue grinding, different tissue: extractant ratios and extraction times were examined to determine their effects on NO3-N recovery. Partitioning data confirmed previous findings which identified the basal stem between ground level and the seed prior to jointing and the 5 cm of stem just above ground level thereafter as the plant parts showing the greatest accumulation of NO3-N. Therefore, these plant parts have the greatest potential as indicators of the N nutritional status of durum spring wheat. Optimal recovery of tissue NO3-N was obtained when stem tissue was separated immediately in the field and dried within 8 hours of sampling, ground to pass a 0.55 mm mesh screen, and extracted for at least 30 minutes when using a sample size of 0.1000 g in conjunction with 25 ml of extractant (i.e. 1:250 plant tissue to extractant ratio).
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U2 - 10.1080/00103628909368104
DO - 10.1080/00103628909368104
M3 - Article
AN - SCOPUS:7144255626
SN - 0010-3624
VL - 20
SP - 607
EP - 622
JO - Communications in Soil Science and Plant Analysis
JF - Communications in Soil Science and Plant Analysis
IS - 5-6
ER -