@article{7aef80697552498292a418d73f79245d,
title = "PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression",
abstract = "Phosphatidylinositol glycan anchor biosynthesis class N (PIGN) has been linked to the suppression of chromosomal instability. The spindle assembly checkpoint complex is responsible for proper chromosome segregation during mitosis to prevent chromosomal instability. In this study, the novel role of PIGN as a regulator of the spindle assembly checkpoint was unveiled in leukemic patient cells and cell lines. Transient downregulation or ablation of PIGN resulted in impaired mitotic checkpoint activation due to the dysregulated expression of spindle assembly checkpoint-related proteins including MAD1, MAD2, BUBR1, and MPS1. Moreover, ectopic overexpression of PIGN restored the expression of MAD2. PIGN regulated the spindle assembly checkpoint by forming a complex with the spindle assembly checkpoint proteins MAD1, MAD2, and the mitotic kinase MPS1. Thus, PIGN could play a vital role in the spindle assembly checkpoint to suppress chromosomal instability associated with leukemic transformation and progression.",
author = "Teye, {Emmanuel K.} and Shasha Lu and Fangyuan Chen and Wenrui Yang and Thomas Abraham and Stairs, {Douglas B.} and Wang, {Hong Gang} and Yochum, {Gregory S.} and Brodsky, {Robert A.} and Pu, {Jeffrey J.}",
note = "Funding Information: The authors thank the patients and their families, nurses, and medical staff for contributing invaluable knowledge and experience to this study. We are indebted to Drs. Taroh Kinoshita, Yoshiko Murakami, and their research team at the Research Institute for Microbial Diseases, Osaka University for providing the HEK293 CRISPR KO cells and pMEPURO3HAhPIGN expression plasmids. This study was supported by AA&MDSIF research grant to JJP (146818), American Cancer Society grant to JJP (124171-IRG-13-043-02), NIDA/FDA research grant to JJP (P50 DA036107), JTTai&Co Foundation Cancer Research Grant to JJP, and a Pennsylvania State University College of Medicine research grant to JJP. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-98218-y",
language = "English (US)",
volume = "11",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}