Physiological Methods

Raina M. Maier; Terry J. Gentry

doi:10.1016/B978-0-12-394626-3.00011-9

Physiological Methods

Raina M. Maier, Terry J. Gentry

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Environmental microbiologists are not only interested in "who" is in the community, but also "what activities" the community carries out. Such activities can be identified and quantified via "physiological methods." The goal of this chapter is to examine different types of microbial activity measurements in both pure culture and environmental samples. In pure culture, activity measurements include: measurement of substrate disappearance; terminal electron acceptor (TEA) utilization; cell mass increase; and carbon dioxide evolution. Environmental activity measurements are discussed in terms of: carbon respiration; incorporation of radiolabeled tracers; adenylate energy determination; enzymatic assays; and stable isotope probing.

Original language	English (US)
Title of host publication	Environmental Microbiology
Subtitle of host publication	Third Edition
Publisher	Elsevier Inc.
Pages	213-243
Number of pages	31
ISBN (Print)	9780123946263
DOIs	https://doi.org/10.1016/B978-0-12-394626-3.00011-9
State	Published - 2015

Keywords

ATP assays
Microbial carbon respiration
Microbial cell mass
Microbial substrate utilization
Microbial terminal electron acceptors
Physiological methods
Radiolabeled tracers

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/B978-0-12-394626-3.00011-9

Cite this

@inbook{a251753b84fa4d8484a8a8883b53aa95,

title = "Physiological Methods",

abstract = "Environmental microbiologists are not only interested in {"}who{"} is in the community, but also {"}what activities{"} the community carries out. Such activities can be identified and quantified via {"}physiological methods.{"} The goal of this chapter is to examine different types of microbial activity measurements in both pure culture and environmental samples. In pure culture, activity measurements include: measurement of substrate disappearance; terminal electron acceptor (TEA) utilization; cell mass increase; and carbon dioxide evolution. Environmental activity measurements are discussed in terms of: carbon respiration; incorporation of radiolabeled tracers; adenylate energy determination; enzymatic assays; and stable isotope probing.",

keywords = "ATP assays, Microbial carbon respiration, Microbial cell mass, Microbial substrate utilization, Microbial terminal electron acceptors, Physiological methods, Radiolabeled tracers",

author = "Maier, {Raina M.} and Gentry, {Terry J.}",

year = "2015",

doi = "10.1016/B978-0-12-394626-3.00011-9",

language = "English (US)",

isbn = "9780123946263",

pages = "213--243",

booktitle = "Environmental Microbiology",

publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Physiological Methods

AU - Maier, Raina M.

AU - Gentry, Terry J.

PY - 2015

Y1 - 2015

N2 - Environmental microbiologists are not only interested in "who" is in the community, but also "what activities" the community carries out. Such activities can be identified and quantified via "physiological methods." The goal of this chapter is to examine different types of microbial activity measurements in both pure culture and environmental samples. In pure culture, activity measurements include: measurement of substrate disappearance; terminal electron acceptor (TEA) utilization; cell mass increase; and carbon dioxide evolution. Environmental activity measurements are discussed in terms of: carbon respiration; incorporation of radiolabeled tracers; adenylate energy determination; enzymatic assays; and stable isotope probing.

AB - Environmental microbiologists are not only interested in "who" is in the community, but also "what activities" the community carries out. Such activities can be identified and quantified via "physiological methods." The goal of this chapter is to examine different types of microbial activity measurements in both pure culture and environmental samples. In pure culture, activity measurements include: measurement of substrate disappearance; terminal electron acceptor (TEA) utilization; cell mass increase; and carbon dioxide evolution. Environmental activity measurements are discussed in terms of: carbon respiration; incorporation of radiolabeled tracers; adenylate energy determination; enzymatic assays; and stable isotope probing.

KW - ATP assays

KW - Microbial carbon respiration

KW - Microbial cell mass

KW - Microbial substrate utilization

KW - Microbial terminal electron acceptors

KW - Physiological methods

KW - Radiolabeled tracers

UR - http://www.scopus.com/inward/record.url?scp=85018828546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018828546&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-394626-3.00011-9

DO - 10.1016/B978-0-12-394626-3.00011-9

M3 - Chapter

AN - SCOPUS:85018828546

SN - 9780123946263

SP - 213

EP - 243

BT - Environmental Microbiology

PB - Elsevier Inc.

ER -

Physiological Methods

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this