Physiologic alterations in the murine model after nasal fungal antigenic exposure

Ayesha N. Khalid; Bradford A. Woodworth; Anthony Prince; Sadeq A. Quraishi; Marcelo B. Antunes; Fenella H.A. Long; William E. Bolger; Alexander G. Chiu; James N. Palmer; Noam A. Cohen

doi:10.1016/j.otohns.2008.07.018

Physiologic alterations in the murine model after nasal fungal antigenic exposure

Ayesha N. Khalid, Bradford A. Woodworth, Anthony Prince, Sadeq A. Quraishi, Marcelo B. Antunes, Fenella H.A. Long, William E. Bolger, Alexander G. Chiu, James N. Palmer, Noam A. Cohen

Surgery

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Objective: To determine whether a recently developed murine model of fungus-induced sinonasal inflammation demonstrated alterations in ciliary activity and expression of inflammatory cytokines. Study Design: A prospective randomized controlled study of rhinosinusitis after fungal antigenic sensitization was performed with intraperitoneal aspergillus antigen injection followed by intranasal antigen challenge for 4 weeks. Saline solution was used in a parallel fashion for control animals. Subjects and Methods: Six mice were used to validate the model. Additional 15 mice were used for ciliary beat frequency (CBF) analysis and cytokine expression with multiplex technology. Mean values for degree of inflammation, secretory hyperplasia, CBF, and cytokine expression were compared. Results: Histologic analyses demonstrated dense chronic inflammation in aspergillus-challenged animals versus sparse inflammatory cells in controls. Significant differences in mean of aspergillus-challenged versus control animals were observed in degree of inflammation (P < 0.01), secretory hyperplasia (P < 0.01), CBF (P < 0.00002), IL-1α (P < 0.0002), IL-1β (P < 0.0003), IL-4 (P < 0.02), TNF-α (P < 0.02), and RANTES (P < 0.01). Conclusion: Alteration in baseline CBF accompanied by increased expression of specific inflammatory cytokines was observed in aspergillus-challenged mice.

Original language	English (US)
Pages (from-to)	695-701
Number of pages	7
Journal	Otolaryngology - Head and Neck Surgery
Volume	139
Issue number	5
DOIs	https://doi.org/10.1016/j.otohns.2008.07.018
State	Published - Nov 2008

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

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10.1016/j.otohns.2008.07.018

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@article{914dbc66c6244b40a9757e63fb015449,

title = "Physiologic alterations in the murine model after nasal fungal antigenic exposure",

abstract = "Objective: To determine whether a recently developed murine model of fungus-induced sinonasal inflammation demonstrated alterations in ciliary activity and expression of inflammatory cytokines. Study Design: A prospective randomized controlled study of rhinosinusitis after fungal antigenic sensitization was performed with intraperitoneal aspergillus antigen injection followed by intranasal antigen challenge for 4 weeks. Saline solution was used in a parallel fashion for control animals. Subjects and Methods: Six mice were used to validate the model. Additional 15 mice were used for ciliary beat frequency (CBF) analysis and cytokine expression with multiplex technology. Mean values for degree of inflammation, secretory hyperplasia, CBF, and cytokine expression were compared. Results: Histologic analyses demonstrated dense chronic inflammation in aspergillus-challenged animals versus sparse inflammatory cells in controls. Significant differences in mean of aspergillus-challenged versus control animals were observed in degree of inflammation (P < 0.01), secretory hyperplasia (P < 0.01), CBF (P < 0.00002), IL-1α (P < 0.0002), IL-1β (P < 0.0003), IL-4 (P < 0.02), TNF-α (P < 0.02), and RANTES (P < 0.01). Conclusion: Alteration in baseline CBF accompanied by increased expression of specific inflammatory cytokines was observed in aspergillus-challenged mice.",

author = "Khalid, {Ayesha N.} and Woodworth, {Bradford A.} and Anthony Prince and Quraishi, {Sadeq A.} and Antunes, {Marcelo B.} and Long, {Fenella H.A.} and Bolger, {William E.} and Chiu, {Alexander G.} and Palmer, {James N.} and Cohen, {Noam A.}",

note = "Funding Information: Research was supported by a Research in Otolaryngology and Allergy Development (ROAD) Scholarship (ANK) and a Young Investigator Award from The Sinus and Allergy Health Partnership (NAC). This work was presented at the Annual Meeting of the American Rhinologic Society and the Academy of Otolaryngic Allergy (AAOA), September 2007, in Washington, DC.",

year = "2008",

month = nov,

doi = "10.1016/j.otohns.2008.07.018",

language = "English (US)",

volume = "139",

pages = "695--701",

journal = "Otolaryngology - Head and Neck Surgery",

issn = "0194-5998",

publisher = "John Wiley and Sons Inc.",

number = "5",

}

TY - JOUR

T1 - Physiologic alterations in the murine model after nasal fungal antigenic exposure

AU - Khalid, Ayesha N.

AU - Woodworth, Bradford A.

AU - Prince, Anthony

AU - Quraishi, Sadeq A.

AU - Antunes, Marcelo B.

AU - Long, Fenella H.A.

AU - Bolger, William E.

AU - Chiu, Alexander G.

AU - Palmer, James N.

AU - Cohen, Noam A.

N1 - Funding Information: Research was supported by a Research in Otolaryngology and Allergy Development (ROAD) Scholarship (ANK) and a Young Investigator Award from The Sinus and Allergy Health Partnership (NAC). This work was presented at the Annual Meeting of the American Rhinologic Society and the Academy of Otolaryngic Allergy (AAOA), September 2007, in Washington, DC.

PY - 2008/11

Y1 - 2008/11

N2 - Objective: To determine whether a recently developed murine model of fungus-induced sinonasal inflammation demonstrated alterations in ciliary activity and expression of inflammatory cytokines. Study Design: A prospective randomized controlled study of rhinosinusitis after fungal antigenic sensitization was performed with intraperitoneal aspergillus antigen injection followed by intranasal antigen challenge for 4 weeks. Saline solution was used in a parallel fashion for control animals. Subjects and Methods: Six mice were used to validate the model. Additional 15 mice were used for ciliary beat frequency (CBF) analysis and cytokine expression with multiplex technology. Mean values for degree of inflammation, secretory hyperplasia, CBF, and cytokine expression were compared. Results: Histologic analyses demonstrated dense chronic inflammation in aspergillus-challenged animals versus sparse inflammatory cells in controls. Significant differences in mean of aspergillus-challenged versus control animals were observed in degree of inflammation (P < 0.01), secretory hyperplasia (P < 0.01), CBF (P < 0.00002), IL-1α (P < 0.0002), IL-1β (P < 0.0003), IL-4 (P < 0.02), TNF-α (P < 0.02), and RANTES (P < 0.01). Conclusion: Alteration in baseline CBF accompanied by increased expression of specific inflammatory cytokines was observed in aspergillus-challenged mice.

AB - Objective: To determine whether a recently developed murine model of fungus-induced sinonasal inflammation demonstrated alterations in ciliary activity and expression of inflammatory cytokines. Study Design: A prospective randomized controlled study of rhinosinusitis after fungal antigenic sensitization was performed with intraperitoneal aspergillus antigen injection followed by intranasal antigen challenge for 4 weeks. Saline solution was used in a parallel fashion for control animals. Subjects and Methods: Six mice were used to validate the model. Additional 15 mice were used for ciliary beat frequency (CBF) analysis and cytokine expression with multiplex technology. Mean values for degree of inflammation, secretory hyperplasia, CBF, and cytokine expression were compared. Results: Histologic analyses demonstrated dense chronic inflammation in aspergillus-challenged animals versus sparse inflammatory cells in controls. Significant differences in mean of aspergillus-challenged versus control animals were observed in degree of inflammation (P < 0.01), secretory hyperplasia (P < 0.01), CBF (P < 0.00002), IL-1α (P < 0.0002), IL-1β (P < 0.0003), IL-4 (P < 0.02), TNF-α (P < 0.02), and RANTES (P < 0.01). Conclusion: Alteration in baseline CBF accompanied by increased expression of specific inflammatory cytokines was observed in aspergillus-challenged mice.

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DO - 10.1016/j.otohns.2008.07.018

M3 - Article

C2 - 18984266

AN - SCOPUS:54549110117

SN - 0194-5998

VL - 139

SP - 695

EP - 701

JO - Otolaryngology - Head and Neck Surgery

JF - Otolaryngology - Head and Neck Surgery

IS - 5

ER -

Physiologic alterations in the murine model after nasal fungal antigenic exposure

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