Physical linkage of the constant region genes for immunoglobulins λ(I) and λ(III)

J. Miller, A. Bothwell, U. Storb

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During differentiation from a stem cell to an antibody-secreting cell, the immunoglobulin genes within a B cell undergo a rearrangement that juxtaposes a variable region gene to a constant region gene. To analyze the genetic organization of an immunoglobulin gene family in nonrearranged, germ-line DNA, we have constructed a recombinant DNA library from randomly cleaved mouse kidney DNA fragments. From this library, we have isolated three overlapping recombinant clones containing the constant region gene for λ(I) light chains (C(λI)). These clones spanned 24.9 kilobases of mouse DNA and contained no variable region sequences. Hybridization of these clones with λ(II) cDNA demonstrated the presence of an additional constant region gene and a joining region 3.2 kilobases 5' of C(λI). This gene was tentatively identified as C(λIII) by the absence of an Ava I endonuclease site, which is present within C(λII). The C(λIII) amino acid sequence has recently been reported and is very closely related to the C(λII) amino acid sequence.

Original languageEnglish (US)
Pages (from-to)3829-3833
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6 I
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

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