Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis

Allan Heres; Donald V. Lightner

doi:10.1016/j.jip.2009.10.004

Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis

Allan Heres, Donald V. Lightner

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

Original language	English (US)
Pages (from-to)	30-35
Number of pages	6
Journal	Journal of Invertebrate Pathology
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/j.jip.2009.10.004
State	Published - Jan 2010

Keywords

Multilocus
Penaeus vannamei
Phylogenetic analysis
Spiroplasma penaei

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/j.jip.2009.10.004

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title = "Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis",

abstract = "A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.",

keywords = "Multilocus, Penaeus vannamei, Phylogenetic analysis, Spiroplasma penaei",

author = "Allan Heres and Lightner, {Donald V.}",

note = "Funding Information: We thank Tamara S. Haselkorn, Bonnie T. Poulos, and Kathy F.J. Tang-Nelson for helpful comments on the manuscript. Funding for this research was provided by a grant from the United States Marine Shrimp Farming Program, United States Department of Agriculture , and Cooperative State Research Education and Extension Service , Grant number 2004-38808-02142 .",

year = "2010",

month = jan,

doi = "10.1016/j.jip.2009.10.004",

language = "English (US)",

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AU - Heres, Allan

AU - Lightner, Donald V.

N1 - Funding Information: We thank Tamara S. Haselkorn, Bonnie T. Poulos, and Kathy F.J. Tang-Nelson for helpful comments on the manuscript. Funding for this research was provided by a grant from the United States Marine Shrimp Farming Program, United States Department of Agriculture , and Cooperative State Research Education and Extension Service , Grant number 2004-38808-02142 .

PY - 2010/1

Y1 - 2010/1

N2 - A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

AB - A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

KW - Multilocus

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KW - Phylogenetic analysis

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Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis

Abstract

Keywords

ASJC Scopus subject areas

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