TY - JOUR
T1 - Photonic Technologies for Liquid Biopsies
T2 - Recent Advances and Open Research Challenges
AU - Dell'Olio, Francesco
AU - Su, Judith
AU - Huser, Thomas
AU - Sottile, Virginie
AU - Cortés-Hernández, Luis Enrique
AU - Alix-Panabières, Catherine
N1 - Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2021/1
Y1 - 2021/1
N2 - The recent development of sophisticated techniques capable of detecting extremely low concentrations of circulating tumor biomarkers in accessible body fluids, such as blood or urine, could contribute to a paradigm shift in cancer diagnosis and treatment. By applying such techniques, clinicians can carry out liquid biopsies, providing information on tumor presence, evolution, and response to therapy. The implementation of biosensing platforms for liquid biopsies is particularly complex because this application domain demands high selectivity/specificity and challenging limit-of-detection (LoD) values. The interest in photonics as an enabling technology for liquid biopsies is growing owing to the well-known advantages of photonic biosensors over competing technologies in terms of compactness, immunity to external disturbance, and ultrahigh spatial resolution. Some encouraging experimental results in the field of photonic devices and systems for liquid biopsy have already been achieved by using fluorescent labels and label-free techniques and by exploiting super-resolution microscopy, surface plasmon resonance, surface-enhanced Raman scattering, and whispering gallery mode resonators. The current state-of-the-art is critically reviewed here, starting from the requirements imposed by the detection of the most common circulating biomarkers. Open research challenges are considered together with competing technologies, and the most promising paths of improvement are discussed for future applications.
AB - The recent development of sophisticated techniques capable of detecting extremely low concentrations of circulating tumor biomarkers in accessible body fluids, such as blood or urine, could contribute to a paradigm shift in cancer diagnosis and treatment. By applying such techniques, clinicians can carry out liquid biopsies, providing information on tumor presence, evolution, and response to therapy. The implementation of biosensing platforms for liquid biopsies is particularly complex because this application domain demands high selectivity/specificity and challenging limit-of-detection (LoD) values. The interest in photonics as an enabling technology for liquid biopsies is growing owing to the well-known advantages of photonic biosensors over competing technologies in terms of compactness, immunity to external disturbance, and ultrahigh spatial resolution. Some encouraging experimental results in the field of photonic devices and systems for liquid biopsy have already been achieved by using fluorescent labels and label-free techniques and by exploiting super-resolution microscopy, surface plasmon resonance, surface-enhanced Raman scattering, and whispering gallery mode resonators. The current state-of-the-art is critically reviewed here, starting from the requirements imposed by the detection of the most common circulating biomarkers. Open research challenges are considered together with competing technologies, and the most promising paths of improvement are discussed for future applications.
KW - liquid biopsies
KW - oncologyoptical microscopy
KW - plasmonics
KW - whispering gallery modes
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U2 - 10.1002/lpor.202000255
DO - 10.1002/lpor.202000255
M3 - Review article
AN - SCOPUS:85097010169
SN - 1863-8880
VL - 15
JO - Laser and Photonics Reviews
JF - Laser and Photonics Reviews
IS - 1
M1 - 2000255
ER -