TY - JOUR
T1 - Photoaffinity labeling of human platelet and rabbit kidney α2-adrenoceptors with [3H]SKF 102229
AU - Regan, John W.
AU - Raymond, John R.
AU - Lefkowitz, Robert J.
AU - DeMarinis, Robert M.
PY - 1986/6/13
Y1 - 1986/6/13
N2 - A newly developed α2-adrenergic photoaffinity ligand, 3-methyl-6-chloro-9-azido-1H-2,3,4,5-tetrahydro-3-benzazepine (SKF 102229), has been radiolabeled with tritium to a specific activity of ∼ 80 Ci/mmol. Using membranes prepared from human platelets and from rabbit kidney, α2-adrenoceptors have been covalently labeled following photolysis in the presence of [3H]SKF 102229. As determined by SDS-PAGE, the apparent molecular weight of α2-adrenoceptors from both of these tissues was 64,000. The yield of covalent insertion of [3H]SKF 102229 into the α2-adrenoceptor was very good. Thus, following photolysis up to 90% of the α2-adrenoceptors could be irreversibly labeled with [3H]SKF 102229.
AB - A newly developed α2-adrenergic photoaffinity ligand, 3-methyl-6-chloro-9-azido-1H-2,3,4,5-tetrahydro-3-benzazepine (SKF 102229), has been radiolabeled with tritium to a specific activity of ∼ 80 Ci/mmol. Using membranes prepared from human platelets and from rabbit kidney, α2-adrenoceptors have been covalently labeled following photolysis in the presence of [3H]SKF 102229. As determined by SDS-PAGE, the apparent molecular weight of α2-adrenoceptors from both of these tissues was 64,000. The yield of covalent insertion of [3H]SKF 102229 into the α2-adrenoceptor was very good. Thus, following photolysis up to 90% of the α2-adrenoceptors could be irreversibly labeled with [3H]SKF 102229.
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U2 - 10.1016/0006-291X(86)91121-6
DO - 10.1016/0006-291X(86)91121-6
M3 - Article
C2 - 3015124
AN - SCOPUS:0022486880
SN - 0006-291X
VL - 137
SP - 606
EP - 613
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -