Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

Jiaqi Shi; John W.B. Hershey; Mark A. Nelson

doi:10.1016/j.febslet.2009.02.028

Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

Jiaqi Shi, John W.B. Hershey, Mark A. Nelson

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46 Scopus citations

Abstract

eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11^p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11^p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11^p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

Original language	English (US)
Pages (from-to)	971-977
Number of pages	7
Journal	FEBS Letters
Volume	583
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2009.02.028
State	Published - Mar 18 2009

Keywords

Apoptosis
Cyclin-dependent kinase 11
Melanoma
Phosphorylation
Translation initiation
eIF3f

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.02.028

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@article{d307a7eb52c04d1ca9173b48bc59aad5,

title = "Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis",

abstract = "eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).",

keywords = "Apoptosis, Cyclin-dependent kinase 11, Melanoma, Phosphorylation, Translation initiation, eIF3f",

author = "Jiaqi Shi and Hershey, {John W.B.} and Nelson, {Mark A.}",

note = "Funding Information: We thank Dr. Ronald Heimark for his support. We thank Dr. George Tsaprailis of the SWEHSC/AZCC proteomics core at the University of Arizona for performing the MS. We also thank the flow cytometry core at the University of Arizona for the apoptosis assay. This work was supported by Cancer Biology Training Grant CA 09213, Faculty Small Grant Program (University of Arizona), ABRC Grant (0007), Grants CA133449 (J.S.), GI SPORE CA095060 (J.S.), CA70145 (M.N.), GM22135 (J.H.), Center Grants ES066694, and NIH Arizona Cancer Center Support Grant CA023074.",

year = "2009",

month = mar,

day = "18",

doi = "10.1016/j.febslet.2009.02.028",

language = "English (US)",

volume = "583",

pages = "971--977",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "6",

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TY - JOUR

T1 - Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

AU - Shi, Jiaqi

AU - Hershey, John W.B.

AU - Nelson, Mark A.

N1 - Funding Information: We thank Dr. Ronald Heimark for his support. We thank Dr. George Tsaprailis of the SWEHSC/AZCC proteomics core at the University of Arizona for performing the MS. We also thank the flow cytometry core at the University of Arizona for the apoptosis assay. This work was supported by Cancer Biology Training Grant CA 09213, Faculty Small Grant Program (University of Arizona), ABRC Grant (0007), Grants CA133449 (J.S.), GI SPORE CA095060 (J.S.), CA70145 (M.N.), GM22135 (J.H.), Center Grants ES066694, and NIH Arizona Cancer Center Support Grant CA023074.

PY - 2009/3/18

Y1 - 2009/3/18

N2 - eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

AB - eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

KW - Apoptosis

KW - Cyclin-dependent kinase 11

KW - Melanoma

KW - Phosphorylation

KW - Translation initiation

KW - eIF3f

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -

Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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