Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation

Eva V. Varga; Dagmar Stropova; Marc Rubenzik; Sue Waite; William R. Roeske; Henry I. Yamamura

doi:10.1016/S0014-2999(98)00847-4

Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation

Eva V. Varga, Dagmar Stropova, Marc Rubenzik, Sue Waite, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

An immunoprecipitation method was used to measure [³²P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human δ-opioid receptor. Chronic SNC 80 ((+)-4-[(αR)-α-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethyl-benzamide) (1 μM, 24 h) treatment increased the incorporation of [³²P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 μM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	R1-R3
Journal	European Journal of Pharmacology
Volume	364
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-2999(98)00847-4
State	Published - Jan 8 1999

Keywords

Adenylyl cyclase VI
Opioid receptor
Phosphorylation

ASJC Scopus subject areas

Pharmacology

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10.1016/S0014-2999(98)00847-4

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title = "Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation",

abstract = "An immunoprecipitation method was used to measure [32P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human δ-opioid receptor. Chronic SNC 80 ((+)-4-[(αR)-α-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethyl-benzamide) (1 μM, 24 h) treatment increased the incorporation of [32P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 μM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Adenylyl cyclase VI, Opioid receptor, Phosphorylation",

author = "Varga, {Eva V.} and Dagmar Stropova and Marc Rubenzik and Sue Waite and Roeske, {William R.} and Yamamura, {Henry I.}",

note = "Funding Information: This work was supported in part from a grant from the Arizona Disease Control Research Commission.",

year = "1999",

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doi = "10.1016/S0014-2999(98)00847-4",

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T1 - Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation

AU - Varga, Eva V.

AU - Stropova, Dagmar

AU - Rubenzik, Marc

AU - Waite, Sue

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: This work was supported in part from a grant from the Arizona Disease Control Research Commission.

PY - 1999/1/8

Y1 - 1999/1/8

N2 - An immunoprecipitation method was used to measure [32P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human δ-opioid receptor. Chronic SNC 80 ((+)-4-[(αR)-α-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethyl-benzamide) (1 μM, 24 h) treatment increased the incorporation of [32P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 μM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide. Copyright (C) 1999 Elsevier Science B.V.

AB - An immunoprecipitation method was used to measure [32P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human δ-opioid receptor. Chronic SNC 80 ((+)-4-[(αR)-α-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethyl-benzamide) (1 μM, 24 h) treatment increased the incorporation of [32P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 μM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide. Copyright (C) 1999 Elsevier Science B.V.

KW - Adenylyl cyclase VI

KW - Opioid receptor

KW - Phosphorylation

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AN - SCOPUS:0032899678

SN - 0014-2999

VL - 364

SP - R1-R3

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2-3

ER -

Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation

Abstract

Keywords

ASJC Scopus subject areas

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