Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads

Jean Vion-Dury; Jean Philippe Galons; Jacques Fantini; Sylviane Confort-Gouny; Mostafa Kriat; Patrick J. Cozzone

doi:10.1016/0006-8993(89)91013-5

Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads

Jean Vion-Dury, Jean Philippe Galons, Jacques Fantini, Sylviane Confort-Gouny, Mostafa Kriat, Patrick J. Cozzone

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.

Original language	English (US)
Pages (from-to)	175-178
Number of pages	4
Journal	Brain Research
Volume	493
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)91013-5
State	Published - Jul 24 1989
Externally published	Yes

Keywords

Cell culture
Glioma cell line
Microcarrier
Nuclear magnetic resonance spectrocopy
P-
Phosphorylated compound

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(89)91013-5

Cite this

@article{1bbc5b8914e542f997df6f3de195e24a,

title = "Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads",

abstract = "The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.",

keywords = "Cell culture, Glioma cell line, Microcarrier, Nuclear magnetic resonance spectrocopy, P-, Phosphorylated compound",

author = "Jean Vion-Dury and Galons, {Jean Philippe} and Jacques Fantini and Sylviane Confort-Gouny and Mostafa Kriat and Cozzone, {Patrick J.}",

note = "Funding Information: This work is supported by C.N.R.S. (Unit6 As-soci6e no. 1186), the Ligue Nationale Contre le Cancer, and the Association pour la Recherche sur le Cancer. J.V.D. is recipient of a fellowship from the Fondation pour la Recherche M6dicale.",

year = "1989",

month = jul,

day = "24",

doi = "10.1016/0006-8993(89)91013-5",

language = "English (US)",

volume = "493",

pages = "175--178",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads

AU - Vion-Dury, Jean

AU - Galons, Jean Philippe

AU - Fantini, Jacques

AU - Confort-Gouny, Sylviane

AU - Kriat, Mostafa

AU - Cozzone, Patrick J.

N1 - Funding Information: This work is supported by C.N.R.S. (Unit6 As-soci6e no. 1186), the Ligue Nationale Contre le Cancer, and the Association pour la Recherche sur le Cancer. J.V.D. is recipient of a fellowship from the Fondation pour la Recherche M6dicale.

PY - 1989/7/24

Y1 - 1989/7/24

N2 - The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.

AB - The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.

KW - Cell culture

KW - Glioma cell line

KW - Microcarrier

KW - Nuclear magnetic resonance spectrocopy

KW - P-

KW - Phosphorylated compound

UR - http://www.scopus.com/inward/record.url?scp=0024379096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024379096&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(89)91013-5

DO - 10.1016/0006-8993(89)91013-5

M3 - Article

C2 - 2776005

AN - SCOPUS:0024379096

SN - 0006-8993

VL - 493

SP - 175

EP - 178

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this