Abstract
The present studies were designed to investigate phosphate transport across the brush border and basolateral membranes of enterocytes and to determine the effect of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] on these processes in suckling and adolescent rats. Vitamin D deficiency was induced in suckling rats by feeding pregnant dams a vitamin D-deficient diet 48 h after insemination; they were then kept in the dark. Vitamin D deficiency in the adolescent rats was induced by feeding the vitamin D-deficient diet to weanling rats for 4 wk. Vmax values for Na+-dependent phosphate uptake in the brush border membranes of vitamin D-deficient and 1,25(OH)2D3-injected suckling rats was 0.7 ± 0.1 and 1.5 ± 0.2 nmol · mg protein-1 · 10 s-1 (P < 0.01), respectively; Vmax values in adolescent rats were 0.2 ± 0.05 and 0.36 ± 0.04 nmol · mg protein-1 · 10 s-1 (P < 0.05), respectively. Vmax values for Na+-dependent phosphate uptake in basolateral membranes of vitamin D-deficient and 1,25(OH)2 D3-treated suckling rats were 0.006 ± 0.001 and 0.047 ± 0.006 nmol · mg protein-1 · 10 s-1 (P < 0.01).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 873-877 |
| Number of pages | 5 |
| Journal | American Journal of Clinical Nutrition |
| Volume | 55 |
| Issue number | 4 |
| State | Published - Apr 1992 |
| Externally published | Yes |
Keywords
- Enterocyte
- Phosphate
- Vitamin D
ASJC Scopus subject areas
- Nutrition and Dietetics
- Medicine (miscellaneous)