Phosphate transport by basolateral plasma membranes of human small intestine

Kazuhiro Kikuchi; Fayez K. Ghishan

doi:10.1016/0016-5085(87)90321-0

Phosphate transport by basolateral plasma membranes of human small intestine

Kazuhiro Kikuchi, Fayez K. Ghishan

Research output: Contribution to journal › Article › peer-review

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Abstract

The characteristics of phosphate transport across the human intestinal basolateral membrane were determined using an enriched preparation in which uphill Na⁺-dependent d-glucose transport could not be demonstrated but adenosine triphosphate-dependent calcium transport was present. The human basolateral membrane vesicles were oriented as follows: 64% inside-out vesicles and 36% rightside-out vesicles or sheets, or both. Phosphate transport showed a 3.4-fold transient "overshoot" phenomena in the presence of an inwardly directed sodium gradient. Computerized Michaelis-Menten kinetics for the net active transport component indicated a V_max of 65 ± 5 pmol/mg protein per 8 s and a K_m of 93 ± 15 μM. The transport process was electroneutral. Gramicidin D and transstimulation studies confirmed the presence of a Na⁺-phosphate carrier at the basolateral membrane. Adenosine triphosphate enhanced phosphate uptake, indicating an adenosine triphosphate-dependent phosphate transport process. These studies are the first to determine the presence of a phosphate carrier in human intestinal basolateral membrane.

Original language	English (US)
Pages (from-to)	106-113
Number of pages	8
Journal	Gastroenterology
Volume	93
Issue number	1
DOIs	https://doi.org/10.1016/0016-5085(87)90321-0
State	Published - Jul 1987

ASJC Scopus subject areas

Hepatology
Gastroenterology

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10.1016/0016-5085(87)90321-0

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title = "Phosphate transport by basolateral plasma membranes of human small intestine",

abstract = "The characteristics of phosphate transport across the human intestinal basolateral membrane were determined using an enriched preparation in which uphill Na+-dependent d-glucose transport could not be demonstrated but adenosine triphosphate-dependent calcium transport was present. The human basolateral membrane vesicles were oriented as follows: 64% inside-out vesicles and 36% rightside-out vesicles or sheets, or both. Phosphate transport showed a 3.4-fold transient {"}overshoot{"} phenomena in the presence of an inwardly directed sodium gradient. Computerized Michaelis-Menten kinetics for the net active transport component indicated a Vmax of 65 ± 5 pmol/mg protein per 8 s and a Km of 93 ± 15 μM. The transport process was electroneutral. Gramicidin D and transstimulation studies confirmed the presence of a Na+-phosphate carrier at the basolateral membrane. Adenosine triphosphate enhanced phosphate uptake, indicating an adenosine triphosphate-dependent phosphate transport process. These studies are the first to determine the presence of a phosphate carrier in human intestinal basolateral membrane.",

author = "Kazuhiro Kikuchi and Ghishan, {Fayez K.}",

note = "Funding Information: Received September 30, 1986. Accepted February 9, 1987. Address requests for reprints to: Fayez K. Ghishan, M.D., Vanderbilt University Medical Center, Department of Pediatrics, Division of Gastroenterology, D-4130 Medical Center North Building, Nashville, Tennessee 37232. This work was supported by National Institutes of Health grant ROl DK 33209-04 and NIH NIADDKD DK 26657. K. Kikuchi{\textquoteright}s present address is: Department of Medicine, School of Medicine, Tokai University, Boseidai, Isehara, Kanagawa, 259-11 Japan. 0 1987 by the American Gastroenterological Association 0016-5085/87/$3.50",

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N1 - Funding Information: Received September 30, 1986. Accepted February 9, 1987. Address requests for reprints to: Fayez K. Ghishan, M.D., Vanderbilt University Medical Center, Department of Pediatrics, Division of Gastroenterology, D-4130 Medical Center North Building, Nashville, Tennessee 37232. This work was supported by National Institutes of Health grant ROl DK 33209-04 and NIH NIADDKD DK 26657. K. Kikuchi’s present address is: Department of Medicine, School of Medicine, Tokai University, Boseidai, Isehara, Kanagawa, 259-11 Japan. 0 1987 by the American Gastroenterological Association 0016-5085/87/$3.50

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N2 - The characteristics of phosphate transport across the human intestinal basolateral membrane were determined using an enriched preparation in which uphill Na+-dependent d-glucose transport could not be demonstrated but adenosine triphosphate-dependent calcium transport was present. The human basolateral membrane vesicles were oriented as follows: 64% inside-out vesicles and 36% rightside-out vesicles or sheets, or both. Phosphate transport showed a 3.4-fold transient "overshoot" phenomena in the presence of an inwardly directed sodium gradient. Computerized Michaelis-Menten kinetics for the net active transport component indicated a Vmax of 65 ± 5 pmol/mg protein per 8 s and a Km of 93 ± 15 μM. The transport process was electroneutral. Gramicidin D and transstimulation studies confirmed the presence of a Na+-phosphate carrier at the basolateral membrane. Adenosine triphosphate enhanced phosphate uptake, indicating an adenosine triphosphate-dependent phosphate transport process. These studies are the first to determine the presence of a phosphate carrier in human intestinal basolateral membrane.

AB - The characteristics of phosphate transport across the human intestinal basolateral membrane were determined using an enriched preparation in which uphill Na+-dependent d-glucose transport could not be demonstrated but adenosine triphosphate-dependent calcium transport was present. The human basolateral membrane vesicles were oriented as follows: 64% inside-out vesicles and 36% rightside-out vesicles or sheets, or both. Phosphate transport showed a 3.4-fold transient "overshoot" phenomena in the presence of an inwardly directed sodium gradient. Computerized Michaelis-Menten kinetics for the net active transport component indicated a Vmax of 65 ± 5 pmol/mg protein per 8 s and a Km of 93 ± 15 μM. The transport process was electroneutral. Gramicidin D and transstimulation studies confirmed the presence of a Na+-phosphate carrier at the basolateral membrane. Adenosine triphosphate enhanced phosphate uptake, indicating an adenosine triphosphate-dependent phosphate transport process. These studies are the first to determine the presence of a phosphate carrier in human intestinal basolateral membrane.

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Phosphate transport by basolateral plasma membranes of human small intestine

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