TY - JOUR
T1 - Phenotypic and functional analysis of 1,25-dihydroxyvitamin d3 receptor mediated modulation of the human myeloma cell line rpmi 8226
AU - Rossi, Jean Francois
AU - Durie, Brain G.M.
AU - Duperray, Christophe
AU - Braich, Theodore
AU - Marion, Samuel L.
AU - Wesley Pike, J.
AU - Haussler, Mark R.
AU - Janbon, Charles
AU - Bataille, Regis
PY - 1988/3
Y1 - 1988/3
N2 - Several recent studies have demonstrated the presence of specific receptors for the 1,25-dihydroxyvitamin D3 (calcitriol) in activated normal lymphocytes. By DNA cellulose chromatography, we show evidence of such specific receptors in the human myeloma cell line RPMI 8226. Nanomolar concentrations of 1,25-dihydroxyvitamin D3 reduce the proliferation of RPMI 8226 cells significantly and simultaneously induce the appearance of both new properties and phenotype expression, such as butyrate esterase, enhanced expression of CD20 (Bl), CD15 (Leu-Ml) antigens and X chains, and decreased expression of the PCI antigen using microfluorometric analysis. But such an increased expression of membrane X chains was not associated with an enhanced secretion of X chains. Furthermore, the bone resorbing activity produced normally by RPMI 8226 cells was reduced significantly after 1,25-dihydroxyvitamin D3 treatment. The possible mechanisms and significance of these new functional and phenotypic properties are discussed with respect to the B-cell lineage.
AB - Several recent studies have demonstrated the presence of specific receptors for the 1,25-dihydroxyvitamin D3 (calcitriol) in activated normal lymphocytes. By DNA cellulose chromatography, we show evidence of such specific receptors in the human myeloma cell line RPMI 8226. Nanomolar concentrations of 1,25-dihydroxyvitamin D3 reduce the proliferation of RPMI 8226 cells significantly and simultaneously induce the appearance of both new properties and phenotype expression, such as butyrate esterase, enhanced expression of CD20 (Bl), CD15 (Leu-Ml) antigens and X chains, and decreased expression of the PCI antigen using microfluorometric analysis. But such an increased expression of membrane X chains was not associated with an enhanced secretion of X chains. Furthermore, the bone resorbing activity produced normally by RPMI 8226 cells was reduced significantly after 1,25-dihydroxyvitamin D3 treatment. The possible mechanisms and significance of these new functional and phenotypic properties are discussed with respect to the B-cell lineage.
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M3 - Article
C2 - 2830017
AN - SCOPUS:0023872533
SN - 0008-5472
VL - 48
SP - 1213
EP - 1216
JO - Cancer Research
JF - Cancer Research
IS - 5
ER -