Phenobarbital induces cytosolic acidification in an established liver epithelial cell line

Anna Marie Wysynski; Raul Martinez-Zaguilán; Carl Alden; A. Jay Gandolfi; Robert J. Gillies

doi:10.1016/0378-4274(94)90094-9

Phenobarbital induces cytosolic acidification in an established liver epithelial cell line

Anna Marie Wysynski, Raul Martinez-Zaguilán, Carl Alden, A. Jay Gandolfi, Robert J. Gillies

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Phenobarbital (PB), a long-acting barbiturate, is also a known tumor promoter. The mechanism responsible for the promoting effect of PB has not yet been elucidated. Changes in intracellular pH (pHⁱⁿ) have been associated with both early and late events of multistage carcinogenesis. We conducted this study to evaluate whether PB alters pH homeostasis. Adult rat liver (ARL) epithelial cells were cultured for 48 h on glass coverslips, loaded with the intracellular pH indicator SNARF-1, and perfused with various concentrations of PB to evaluate its effect on pHⁱⁿ. Our results show that PB treatment of cultured cells induces a concentration-dependent cytosolic acidification. These results indicate that its ability to decrease pHⁱⁿ may be involved in the mechanism of tumor promotion by PB.

Original language	English (US)
Pages (from-to)	157-166
Number of pages	10
Journal	Toxicology letters
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/0378-4274(94)90094-9
State	Published - Nov 1994

Keywords

Fluorescence
Intracellular pH
Ion transport
Liver
Liver epithelial cells
Phenobarbital
SNARF-1

ASJC Scopus subject areas

Toxicology

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@article{a5e3440e262e4e9e80e012b0d273f357,

title = "Phenobarbital induces cytosolic acidification in an established liver epithelial cell line",

abstract = "Phenobarbital (PB), a long-acting barbiturate, is also a known tumor promoter. The mechanism responsible for the promoting effect of PB has not yet been elucidated. Changes in intracellular pH (pHin) have been associated with both early and late events of multistage carcinogenesis. We conducted this study to evaluate whether PB alters pH homeostasis. Adult rat liver (ARL) epithelial cells were cultured for 48 h on glass coverslips, loaded with the intracellular pH indicator SNARF-1, and perfused with various concentrations of PB to evaluate its effect on pHin. Our results show that PB treatment of cultured cells induces a concentration-dependent cytosolic acidification. These results indicate that its ability to decrease pHin may be involved in the mechanism of tumor promotion by PB.",

keywords = "Fluorescence, Intracellular pH, Ion transport, Liver, Liver epithelial cells, Phenobarbital, SNARF-1",

author = "Wysynski, {Anna Marie} and Raul Martinez-Zaguil{\'a}n and Carl Alden and Gandolfi, {A. Jay} and Gillies, {Robert J.}",

note = "Funding Information: The authorsw ishto acknowledgeD r. C.A. McQueenf or technicaal ssistancaen d useo f cell culturef acilitiesa nd the AmericanH ealth Foundationf or providingt he ARL epithelialc ells. Supportedb y NIH grant ROl GM43046-01a nd Am. Cant. Sot. grantN o. CN-53 to RJG.",

year = "1994",

month = nov,

doi = "10.1016/0378-4274(94)90094-9",

language = "English (US)",

volume = "74",

pages = "157--166",

journal = "Toxicology letters",

issn = "0378-4274",

publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Phenobarbital induces cytosolic acidification in an established liver epithelial cell line

AU - Wysynski, Anna Marie

AU - Martinez-Zaguilán, Raul

AU - Alden, Carl

AU - Gandolfi, A. Jay

AU - Gillies, Robert J.

N1 - Funding Information: The authorsw ishto acknowledgeD r. C.A. McQueenf or technicaal ssistancaen d useo f cell culturef acilitiesa nd the AmericanH ealth Foundationf or providingt he ARL epithelialc ells. Supportedb y NIH grant ROl GM43046-01a nd Am. Cant. Sot. grantN o. CN-53 to RJG.

PY - 1994/11

Y1 - 1994/11

N2 - Phenobarbital (PB), a long-acting barbiturate, is also a known tumor promoter. The mechanism responsible for the promoting effect of PB has not yet been elucidated. Changes in intracellular pH (pHin) have been associated with both early and late events of multistage carcinogenesis. We conducted this study to evaluate whether PB alters pH homeostasis. Adult rat liver (ARL) epithelial cells were cultured for 48 h on glass coverslips, loaded with the intracellular pH indicator SNARF-1, and perfused with various concentrations of PB to evaluate its effect on pHin. Our results show that PB treatment of cultured cells induces a concentration-dependent cytosolic acidification. These results indicate that its ability to decrease pHin may be involved in the mechanism of tumor promotion by PB.

AB - Phenobarbital (PB), a long-acting barbiturate, is also a known tumor promoter. The mechanism responsible for the promoting effect of PB has not yet been elucidated. Changes in intracellular pH (pHin) have been associated with both early and late events of multistage carcinogenesis. We conducted this study to evaluate whether PB alters pH homeostasis. Adult rat liver (ARL) epithelial cells were cultured for 48 h on glass coverslips, loaded with the intracellular pH indicator SNARF-1, and perfused with various concentrations of PB to evaluate its effect on pHin. Our results show that PB treatment of cultured cells induces a concentration-dependent cytosolic acidification. These results indicate that its ability to decrease pHin may be involved in the mechanism of tumor promotion by PB.

KW - Fluorescence

KW - Intracellular pH

KW - Ion transport

KW - Liver

KW - Liver epithelial cells

KW - Phenobarbital

KW - SNARF-1

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DO - 10.1016/0378-4274(94)90094-9

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C2 - 7940597

AN - SCOPUS:0028037798

SN - 0378-4274

VL - 74

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EP - 166

JO - Toxicology letters

JF - Toxicology letters

IS - 2

ER -

Phenobarbital induces cytosolic acidification in an established liver epithelial cell line

Abstract

Keywords

ASJC Scopus subject areas

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