@article{53bc8bcfc1b040d69dd01ad043fbee24,
title = "pH neutralization protects against reduction in replicative lifespan following chronological aging in yeast",
abstract = "Chronological and replicative aging have been studied in yeast as alternative paradigms for post-mitotic and mitotic aging, respectively. It has been known for more than a decade that cells of the S288C background aged chronologically in rich medium have reduced replicative lifespan relative to chronologically young cells. Here we report replication of this observation in the diploid BY4743 strain background. We further show that the reduction in replicative lifespan from chronological aging is accelerated when cells are chronologically aged under standard conditions in synthetic complete medium rather than rich medium. The loss of replicative potential with chronological age is attenuated by buffering the pH of the chronological aging medium to 6.0, an intervention that we have previously shown can extend chronological lifespan. These data demonstrate that extracellular acidification of the culture medium can cause intracellular damage in the chronologically aging population that is asymmetrically segregated by the mother cell to limit subsequent replicative lifespan.",
keywords = "Chronological lifespan, Longevity, Replication stress, Replicative lifespan, Yeast",
author = "Christopher Murakami and Delaney, {Joe R.} and Annie Chou and Daniel Carr and Jennifer Schleit and Sutphin, {George L.} and An, {Elroy H.} and Castanza, {Anthony S.} and Marissa Fletcher and Sarani Goswami and Sean Higgins and Mollie Holmberg and Jessica Hui and Monika Jelic and Jeong, {Ki Soo} and Kim, {Jin R.} and Shannon Klum and Eric Liao and Lin, {Michael S.} and Winston Lo and Hillary Miller and Richard Moller and Peng, {Zhao J.} and Tom Pollard and Prarthana Pradeep and Dillon Pruett and Dilreet Rai and Vanessa Ros and Alex Schuster and Minnie Singh and Spector, {Benjamin L.} and {Vander Wende}, Helen and Wang, {Adrienne M.} and Wasko, {Brian M.} and Brady Olsen and Matt Kaeberlein",
note = "Funding Information: into the plates and 60–1080 cells (depending on the predicted We would like to thank Donna Prunkard for technical assistance viability of the culture at that point) were randomly selected for with the flow cytometry experiments. This work was supported by RLS analysis. Selected cells were moved to the middle of the a grant from the Ellison Medical Foundation to M.K. Additional YEPD plates, and the RLS of those cells was measured. Statistical support for flow cytometry was provided by the University of significance for lifespan differences was determined using the Washington Nathan Shock Center of Excellence in the Basic Wilcoxon Rank-Sum test. Regression statistics were performed Biology of Aging (NIH P30AG013280). J.R.D. and G.L.S. were using Microsoft Excel Analysis Toolpak{\textquoteright}s “Regression” func-supported by NIH Training Grant T32AG000057. J.S. and tion, and regression lines were compared (Fig. 2) using Microsoft B.M.W. were supported by NIH Training Grant T32ES007032.",
year = "2012",
month = aug,
day = "15",
doi = "10.4161/cc.21465",
language = "English (US)",
volume = "11",
pages = "3087--3096",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Taylor and Francis Ltd.",
number = "16",
}