Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Stephan O. Krause; William G. Gutheil; Anton M. Simeonov; Terry O. Matsunaga; Margaret J. Stauber; George L. Kenyon; Charles E. Mckenna

doi:10.1080/10426509908546294

Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Stephan O. Krause
, William G. Gutheil
, Anton M. Simeonov
, Terry O. Matsunaga
, Margaret J. Stauber
, George L. Kenyon
, Charles E. Mckenna

Research output: Contribution to journal › Review article › peer-review

Abstract

The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [¹⁸O₄]-Pi, b,g-[¹⁸O]-ATP, and g-[¹⁸O₄]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-P_b bond in enzyme-bound ADP.

Original language	English (US)
Pages (from-to)	513-516
Number of pages	4
Journal	Phosphorus, Sulfur and Silicon and Related Elements
Volume	144-146
DOIs	https://doi.org/10.1080/10426509908546294
State	Published - 1999
Externally published	Yes

Keywords

ATP hydrolysis
HRMS
Nitrogenase
P NMR
PIX
[O]-phosphate
g-[O]-ATP

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Inorganic Chemistry

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10.1080/10426509908546294

Cite this

@article{973de98a6f1944b191c20112382e610e,

title = "Perspectives on the mechanism of ATP hydrolysis by nitrogenase",

abstract = "The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.",

keywords = "ATP hydrolysis, HRMS, Nitrogenase, P NMR, PIX, [O]-phosphate, g-[O]-ATP",

author = "Krause, \{Stephan O.\} and Gutheil, \{William G.\} and Simeonov, \{Anton M.\} and Matsunaga, \{Terry O.\} and Stauber, \{Margaret J.\} and Kenyon, \{George L.\} and Mckenna, \{Charles E.\}",

note = "Funding Information: for grants supporting this research.",

year = "1999",

doi = "10.1080/10426509908546294",

language = "English (US)",

volume = "144-146",

pages = "513--516",

journal = "Phosphorus, Sulfur and Silicon and Related Elements",

issn = "1042-6507",

publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Perspectives on the mechanism of ATP hydrolysis by nitrogenase

AU - Krause, Stephan O.

AU - Gutheil, William G.

AU - Simeonov, Anton M.

AU - Matsunaga, Terry O.

AU - Stauber, Margaret J.

AU - Kenyon, George L.

AU - Mckenna, Charles E.

N1 - Funding Information: for grants supporting this research.

PY - 1999

Y1 - 1999

N2 - The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.

AB - The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.

KW - ATP hydrolysis

KW - HRMS

KW - Nitrogenase

KW - P NMR

KW - PIX

KW - [O]-phosphate

KW - g-[O]-ATP

UR - https://www.scopus.com/pages/publications/4243808792

UR - https://www.scopus.com/pages/publications/4243808792#tab=citedBy

U2 - 10.1080/10426509908546294

DO - 10.1080/10426509908546294

M3 - Review article

AN - SCOPUS:4243808792

SN - 1042-6507

VL - 144-146

SP - 513

EP - 516

JO - Phosphorus, Sulfur and Silicon and Related Elements

JF - Phosphorus, Sulfur and Silicon and Related Elements

ER -

Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Abstract

Keywords

ASJC Scopus subject areas

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