Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Stephan O. Krause; William G. Gutheil; Anton M. Simeonov; Terry O. Matsunaga; Margaret J. Stauber; George L. Kenyon; Charles E. Mckenna

doi:10.1080/10426509908546294

Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Stephan O. Krause, William G. Gutheil, Anton M. Simeonov, Terry O. Matsunaga, Margaret J. Stauber, George L. Kenyon, Charles E. Mckenna

Research output: Contribution to journal › Review article › peer-review

Abstract

The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [¹⁸O₄]-Pi, b,g-[¹⁸O]-ATP, and g-[¹⁸O₄]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-P_b bond in enzyme-bound ADP.

Original language	English (US)
Pages (from-to)	513-516
Number of pages	4
Journal	Phosphorus, Sulfur and Silicon and Related Elements
Volume	144-146
DOIs	https://doi.org/10.1080/10426509908546294
State	Published - 1999
Externally published	Yes

Keywords

ATP hydrolysis
HRMS
Nitrogenase
P NMR
PIX
[O]-phosphate
g-[O]-ATP

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Inorganic Chemistry

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10.1080/10426509908546294

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title = "Perspectives on the mechanism of ATP hydrolysis by nitrogenase",

abstract = "The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.",

keywords = "ATP hydrolysis, HRMS, Nitrogenase, P NMR, PIX, [O]-phosphate, g-[O]-ATP",

author = "Krause, {Stephan O.} and Gutheil, {William G.} and Simeonov, {Anton M.} and Matsunaga, {Terry O.} and Stauber, {Margaret J.} and Kenyon, {George L.} and Mckenna, {Charles E.}",

note = "Funding Information: for grants supporting this research.",

year = "1999",

doi = "10.1080/10426509908546294",

language = "English (US)",

volume = "144-146",

pages = "513--516",

journal = "Phosphorus, Sulfur and Silicon and Related Elements",

issn = "1042-6507",

publisher = "Taylor and Francis Ltd.",

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TY - JOUR

T1 - Perspectives on the mechanism of ATP hydrolysis by nitrogenase

AU - Krause, Stephan O.

AU - Gutheil, William G.

AU - Simeonov, Anton M.

AU - Matsunaga, Terry O.

AU - Stauber, Margaret J.

AU - Kenyon, George L.

AU - Mckenna, Charles E.

N1 - Funding Information: for grants supporting this research.

PY - 1999

Y1 - 1999

N2 - The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.

AB - The chemical mechanism of ATP hydrolysis by nitrogenase is discussed in terms of results from isotopic exchange studies using [18O4]-Pi, b,g-[18O]-ATP, and g-[18O4]-ATP as probes of reversibility (ATP = ADP + Pi) and of the freedom of rotation around the O-Pb bond in enzyme-bound ADP.

KW - ATP hydrolysis

KW - HRMS

KW - Nitrogenase

KW - P NMR

KW - PIX

KW - [O]-phosphate

KW - g-[O]-ATP

UR - http://www.scopus.com/inward/record.url?scp=4243808792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4243808792&partnerID=8YFLogxK

U2 - 10.1080/10426509908546294

DO - 10.1080/10426509908546294

M3 - Review article

AN - SCOPUS:4243808792

SN - 1042-6507

VL - 144-146

SP - 513

EP - 516

JO - Phosphorus, Sulfur and Silicon and Related Elements

JF - Phosphorus, Sulfur and Silicon and Related Elements

ER -

Perspectives on the mechanism of ATP hydrolysis by nitrogenase

Abstract

Keywords

ASJC Scopus subject areas

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