TY - JOUR
T1 - Permanent slide preparations of T lymphocyte-sheep red blood cell rosettes
AU - Schafer, Larry A.
AU - Gutterman, Jordan U.
AU - Mavlight, Giora M.
AU - Reed, Richard C.
AU - Hersh, Evan M.
N1 - Funding Information:
In man, circulating thymus-derived lymphocytes (T cells) spontaneously form non-immune rosettes with sheep erythrocytes (SRBC) (FrSland, 1972; Jondal et al., 1972; Papamichael et al., 1972; Wybran et al., 1972; Ross et al., 1973). These rosettes are thought to quantitate circulating T cells in humans (FrSland, 1972; Jondal et al., 1972; Papamichael et al., 1972; Stjernsw~d et al., 1972; Wybran and Fudenberg, 1972; Broome et al., 1973; Farid et al., 1973; Ross et al., 1973; Wybran et al., 1973; Cohnen et al., 1974; Collins et al., 1974; De Horatius et al., 1974; Edelson et al., 1974; Shevach et al., 1974; Zucker-Franklin et al., 1974). At a recent WHO/IARC (World Health Organization/International Association for Research on Cancer) workshop on 'identifying, enumerating, and isolating B and T cells from human peripheral blood', the quantitation of T cell rosettes was considered feasible 'either in suspension or in a fixed and stained preparation' (WHO/IARC special technical report on human T and B cells, 1974). Yet the workshop participants, in their own research, use wet suspensions to enumerate human T cells (FrSland, 1972; Stjernsw~d et al., 1972; Wybran et al., 1972; Bentwich et al., 1973b; Ross et al., 1973; Wybran Supported by Grants CA 05831 and 5-RO1-CA 14984 and contract 33888 from the National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014. Dr. Gutterman and Dr. Mavligit are the recipients, respectively, of career developmental awards (CA 71007-01) and (CA 1 KO 4 CA 00130-01) from the National Institutes of Health.
PY - 1975/9
Y1 - 1975/9
N2 - Rosette formation between sheep erythrocytes (SRBC) and human thymus-derived lymphocytes (T cells) is used to monitor T cells in various human diseases. Rosettes are usually counted in a hemacytometer immediately after preparation. This paper reports a technique for permanently fixing and staining rosettes for sereial and comparative studies, a procedure which has probably been less well standardized, less reproducibly performed, and less widely used than many investigators appreciate. The technique described has the advantages of providing distinct morphological identification of the rosette-forming cell and it produces a permanent mount for future reference.
AB - Rosette formation between sheep erythrocytes (SRBC) and human thymus-derived lymphocytes (T cells) is used to monitor T cells in various human diseases. Rosettes are usually counted in a hemacytometer immediately after preparation. This paper reports a technique for permanently fixing and staining rosettes for sereial and comparative studies, a procedure which has probably been less well standardized, less reproducibly performed, and less widely used than many investigators appreciate. The technique described has the advantages of providing distinct morphological identification of the rosette-forming cell and it produces a permanent mount for future reference.
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U2 - 10.1016/0022-1759(75)90117-9
DO - 10.1016/0022-1759(75)90117-9
M3 - Article
C2 - 1081106
AN - SCOPUS:0016696394
VL - 8
SP - 241
EP - 249
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3
ER -